Overview

  • Product nameV5 tag peptide

Description

  • NatureSynthetic

Specifications

Our Abpromise guarantee covers the use of ab15829 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking - Blocking peptide for Anti-V5 tag antibody (FITC) (ab1209), Anti-V5 tag antibody (Agarose) (ab1229), Anti-V5 tag antibody (FITC) (ab1274), Anti-V5 tag antibody (HRP) (ab1325), Anti-V5 tag antibody (ab9113), Anti-V5 tag antibody - ChIP Grade (ab9116), Anti-V5 tag antibody (ab9137), Anti-V5 tag antibody - ChIP Grade (ab15828)

  • FormLiquid
  • Additional notes

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Information available upon request.

General Info

  • Alternative names
    • GKPIPNPLLGLDST epitope tag
    • GKPIPNPLLGLDST tag
    • Protein Rev
    • Regulator of expression of viral proteins
    • rev
    • V5 epitope tag
    see all
  • RelevanceThe V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).

V5 tag peptide images

  • All lanes : Anti-V5 tag antibody - ChIP Grade (ab15828) at 0.5 µg/ml

    Lane 1 : Recombinant V5 tagged Groucho homolog
    Lane 2 : Recombinant V5 tagged Groucho with V5 tag peptide (ab15829) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat anti-Rabbit IgG at 1/10000 dilution

    Performed under reducing conditions.

    Observed band size : 25 kDa (why is the actual band size different from the predicted?)

References for V5 tag peptide (ab15829)

ab15829 has not yet been referenced specifically in any publications.

Product Wall

I am sorry that this product has been giving results which have made analysis difficult. I am happy to provide the blocking peptide for this product free of charge, so that you might run an immunogen blocking experiment to determine specificity. I hav...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"