Overview

  • Product name
  • Description
    Mouse polyclonal to VAC14
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length VAC14 protein (Human)

  • Positive control
    • Human liver tissue lysate. VAC14 transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab67369 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 88 kDa (predicted molecular weight: 88 kDa).

Target

  • Function
    The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol-3,5-bisphosphate (PtdIns(3,5)P2). Acts as a positive activator of PIKfyve kinase activity. Also required to maintain normal levels of phosphatidylinositol 3-phosphate (PtdIns(3)P) and phosphatidylinositol 5-phosphate (PtdIns(5)P). Plays a role in the biogenesis of endosome carrier vesicles (ECV) / multivesicular bodies (MVB) transport intermediates from early endosomes.
  • Tissue specificity
    Ubiquitously expressed.
  • Sequence similarities
    Belongs to the VAC14 family.
    Contains 6 HEAT repeats.
  • Domain
    The C-terminal domain (residues 523-782) mediates homomeric interactions and is necessary for the formation and maintenance of the PI(3,5)P2 regulatory complex.
  • Cellular localization
    Endosome membrane. Microsome membrane. Mainly associated with membranes of the late endocytic pathway.
  • Information by UniProt
  • Database links
  • Alternative names
    • ArPIKfyve antibody
    • FLJ10305 antibody
    • FLJ36622 antibody
    • FLJ46582 antibody
    • MGC149815 antibody
    • MGC149816 antibody
    • OTTHUMP00000174908 antibody
    • Protein VAC14 homolog antibody
    • Tax1 (human T cell leukemia virus type I) binding protein 1 antibody
    • Tax1 (human T cell leukemia virus type I) binding protein 2 antibody
    • Tax1 binding protein 2 antibody
    • Tax1-binding protein 2 antibody
    • TAX1BP2 antibody
    • TRX antibody
    • VAC 14 antibody
    • vac14 antibody
    • Vac14 homolog (S. cerevisiae) antibody
    • Vac14 homolog antibody
    • VAC14_HUMAN antibody
    see all

Anti-VAC14 antibody images

  • Anti-VAC14 antibody (ab67369) at 1/500 dilution + Human liver tissue lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 88 kDa
    Observed band size : 88 kDa
  • All lanes : Anti-VAC14 antibody (ab67369) at 1/500 dilution

    Lane 1 : VAC14 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 88 kDa
    Observed band size : 88 kDa

References for Anti-VAC14 antibody (ab67369)

ab67369 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Loading amount
40 µg
Specification
HeLa
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 20°C
Username

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Submitted Oct 31 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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