Anti-Vaccinia Virus antibody (ab117453)

Overview

  • Product nameAnti-Vaccinia Virus antibody
    See all Vaccinia Virus primary antibodies
  • Description
    Rabbit polyclonal to Vaccinia Virus
  • Tested applicationsSuitable for: ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Vaccinia Virus
  • Immunogen

    Lister Strain (mixture of virions and infected cell polypeptides)

Properties

Applications

Our Abpromise guarantee covers the use of ab117453 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Target

  • RelevanceVaccinia virus is an Orthopoxvirus, containing double stranded DNA. Fusion protein plays an important role in the entry of enveloped virus into cells. As vaccinia virus has a wide host range, it is conceivable that certain cellular components that are ubiquitously expressed on the cell mediate virus infection. The study of the entry process, attachment, fusion and the proteins and receptors involved is complex. During vaccinia virus infection, the fusion process is attributed to the action of the 14KDa protein (A27L). The N terminus of this protein recognises heparan sulfate on the cell surface. It interacts with the negative charges of sulfates of glycosaminoglycans (GAGs). Therefore, antibodies that recognize this 14KDa protein are able to neutralize vaccinia virus infection and enable identification other viral and cellular proteins which participate in the vaccinia virus entry process.
  • Alternative names
    • A27L antibody
    • Orthopoxvirus antibody

References for Anti-Vaccinia Virus antibody (ab117453)

ab117453 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (MDA-MB-231 tumor)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate pH 6
Specification MDA-MB-231 tumor
Blocking step DAKO blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Fixative Formaldehyde
Username

Mr. Shyambabu Chaurasiya

Verified customer

Submitted Dec 18 2015


Unfortunately, none of the anti-Vaccinia Virus antibodies we have has been tested for cross-reactivity with Western Reserve Strain.


ab117453 has been tested and cross-reacts with 3 other Vaccinia strains and also with distantly relat...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"