Anti-Vaccinia Virus antibody (ab26853)
Key features and details
- Rabbit polyclonal to Vaccinia Virus
- Suitable for: ELISA
- Reacts with: Vaccinia virus
- Isotype: IgG
Overview
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Product name
Anti-Vaccinia Virus antibody
See all Vaccinia Virus primary antibodies -
Description
Rabbit polyclonal to Vaccinia Virus -
Host species
Rabbit -
Tested applications
Suitable for: ELISAmore details -
Species reactivity
Reacts with: Vaccinia virus -
Immunogen
Synthetic peptide corresponding to Vaccinia Virus (N terminal). Containing amino acids on the predicted N terminal of fusion protein (A27L) in vaccinia virus.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Constituent: Whole serum -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab26853 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
1/5000.
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Notes |
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ELISA
1/5000. |
Target
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Relevance
Vaccinia virus is an Orthopoxvirus, containing double stranded DNA. Fusion protein plays an important role in the entry of enveloped virus into cells. As vaccinia virus has a wide host range, it is conceivable that certain cellular components that are ubiquitously expressed on the cell mediate virus infection. The study of the entry process, attachment, fusion and the proteins and receptors involved is complex. During vaccinia virus infection, the fusion process is attributed to the action of the 14KDa protein (A27L). The N terminus of this protein recognises heparan sulfate on the cell surface. It interacts with the negative charges of sulfates of glycosaminoglycans (GAGs). Therefore, antibodies that recognize this 14KDa protein are able to neutralize vaccinia virus infection and enable identification other viral and cellular proteins which participate in the vaccinia virus entry process. -
Alternative names
- Orthopoxvirus antibody
Images
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ELISA method:
Antigen is coated on EIA strips at 1µg per well.
Add 200µl blocking buffer then wash wells with PBST.
Antiserum or peptide specific purified antibody is diluted in series as 102~106 folds.
Incubate antibody for 1 hour.
Wash unbound antibodies and add HRP-conjugated anti-rabbit IgG.
Wash plates and add substrate to develop color for 5 minutes.
Read absorbance at 650nm. Amount of colour is directly proportional to amount of antibodies.
Antibody titer is defined as >0.1 of absorbance of antiserum minus pre-immune serum.
Titer ~ 1/437000.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab26853 has not yet been referenced specifically in any publications.