Overview

  • Product nameAnti-Vaccinia Virus antibody
    See all Vaccinia Virus primary antibodies
  • Description
    Rabbit polyclonal to Vaccinia Virus
  • SpecificityThis antibody reacts with purified Virions. It does not react with uninfected cells.
  • Tested applicationsSuitable for: ELISA, WB, IHC-P, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Does not react with parainfluenza (1-3), RSV, Adeno, Influenza A and B or HSV (1). Reactive with the Lister, Wyeth, New York City and MVA strains of Vaccinia.
  • Immunogen

    Native virus, Lister strain.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purification notesThis antibody is greater than 95% pure.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab35219 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/500 - 1/2000.
WB Use at an assay dependent concentration. Predicted molecular weight: 14 kDa. PubMed: 25093734
IHC-P Use at an assay dependent concentration. PubMed: 25093734
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 22615950

Target

  • RelevanceVaccinia virus is an Orthopoxvirus, containing double stranded DNA. Fusion protein plays an important role in the entry of enveloped virus into cells. As vaccinia virus has a wide host range, it is conceivable that certain cellular components that are ubiquitously expressed on the cell mediate virus infection. The study of the entry process, attachment, fusion and the proteins and receptors involved is complex. During vaccinia virus infection, the fusion process is attributed to the action of the 14KDa protein (A27L). The N terminus of this protein recognises heparan sulfate on the cell surface. It interacts with the negative charges of sulfates of glycosaminoglycans (GAGs). Therefore, antibodies that recognize this 14KDa protein are able to neutralize vaccinia virus infection and enable identification other viral and cellular proteins which participate in the vaccinia virus entry process.
  • Alternative names
    • A27L antibody
    • Orthopoxvirus antibody

References for Anti-Vaccinia Virus antibody (ab35219)

This product has been referenced in:
  • Adelfinger M  et al. Evaluation of a new recombinant oncolytic vaccinia virus strain GLV-5b451 for feline mammary carcinoma therapy. PLoS One 9:e104337 (2014). WB, IHC-P . Read more (PubMed: 25093734) »
  • Weibel S  et al. Treatment of malignant effusion by oncolytic virotherapy in an experimental subcutaneous xenograft model of lung cancer. J Transl Med 11:106 (2013). IHC-Fr . Read more (PubMed: 23635329) »

See all 6 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Cross section of mouse eye, looked at cornea)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TRIS EDTA pH 9
Permeabilization No
Specification Cross section of mouse eye, looked at cornea
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Ms. Inna Larsen

Verified customer

Submitted Dec 29 2015


Unfortunately, none of the anti-Vaccinia Virus antibodies we have has been tested for cross-reactivity with Western Reserve Strain.


ab117453 has been tested and cross-reacts with 3 other Vaccinia strains and also with distantly relat...

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Thank you for contacting us.

We only have two monoclonal antibodies against the vaccinia virus in our catalogue.

I would recommend using ab21098 - Mouse monoclonal [TV46] to Vaccinia Virus (http://www.abcam.com/ab21098) to recognise A...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"