Anti-Vaccinia Virus antibody (Biotin) (ab21039)

Overview

  • Product nameAnti-Vaccinia Virus antibody (Biotin)
    See all Vaccinia Virus primary antibodies
  • Description
    Rabbit polyclonal to Vaccinia Virus (Biotin)
  • ConjugationBiotin
  • SpecificityAb21039 recognizes purified virions and is reactive with Lister, Wyeth, New York City and MVA strains of Vaccinia. Does not react with uninfected cells.
  • Tested applicationsSuitable for: ELISAmore details
  • Species reactivity
    Does not react with Parainfluenza (1-3), RSV, Adenovirus, Influenza A & B or HSV-1. Not yet tested in other species.
  • Immunogen

    Lister Strain (mixture of virions and infected cell polypeptides).

  • General notes

    The antibody is covalently coupled with the N-Hydroxysuccinimide ester of biotin under mild conditions to give a high degree of substitution.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 0.01M PBS, pH 7.2
  • Concentration information loading...
  • PurityIgG fraction
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab21039 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.

Target

  • RelevanceVaccinia virus is an Orthopoxvirus, containing double stranded DNA. Fusion protein plays an important role in the entry of enveloped virus into cells. As vaccinia virus has a wide host range, it is conceivable that certain cellular components that are ubiquitously expressed on the cell mediate virus infection. The study of the entry process, attachment, fusion and the proteins and receptors involved is complex. During vaccinia virus infection, the fusion process is attributed to the action of the 14KDa protein (A27L). The N terminus of this protein recognises heparan sulfate on the cell surface. It interacts with the negative charges of sulfates of glycosaminoglycans (GAGs). Therefore, antibodies that recognize this 14KDa protein are able to neutralize vaccinia virus infection and enable identification other viral and cellular proteins which participate in the vaccinia virus entry process.
  • Alternative names
    • A27L antibody
    • Orthopoxvirus antibody

References for Anti-Vaccinia Virus antibody (Biotin) (ab21039)

ab21039 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. Ab21098 - Mouse monoclonal [TV46] to Vaccinia Virus - reacts with a 27 kDa protein and is weakly reactive with 57 kDa and 6 1kDa proteins in Western blot. The identity of the proteins have not been determined. Ab21039 - ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"