Overview

  • Product nameAnti-Vaccinia Virus antibody (FITC)
    See all Vaccinia Virus primary antibodies
  • Description
    Rabbit polyclonal to Vaccinia Virus (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • SpecificityAb19970 recognizes purified virions.
  • Tested applicationsSuitable for: ICC/IFmore details
  • Species reactivity
    Ab19970 is reactive with Lister, Wyeth, New York City and MVA strains of Vaccinia. Does not react with Parainfluenza (1-3), RSV, Adenovirus, Influenza A&B or HSV-1 or with uninfected cells.
  • Immunogen

    Lister strain (mixture of virions and infected cell polypeptides)

  • General notes

    IgG fraction covalently coupled with high purity Isomer I of fluorescein isothiocyanate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 10mg/ml BSA, 10mM PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notesCare is taken to ensure complete removal of any free fluorescein from the final product.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab19970 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.

Target

  • RelevanceVaccinia virus is an Orthopoxvirus, containing double stranded DNA. Fusion protein plays an important role in the entry of enveloped virus into cells. As vaccinia virus has a wide host range, it is conceivable that certain cellular components that are ubiquitously expressed on the cell mediate virus infection. The study of the entry process, attachment, fusion and the proteins and receptors involved is complex. During vaccinia virus infection, the fusion process is attributed to the action of the 14KDa protein (A27L). The N terminus of this protein recognises heparan sulfate on the cell surface. It interacts with the negative charges of sulfates of glycosaminoglycans (GAGs). Therefore, antibodies that recognize this 14KDa protein are able to neutralize vaccinia virus infection and enable identification other viral and cellular proteins which participate in the vaccinia virus entry process.
  • Alternative names
    • A27L antibody
    • Orthopoxvirus antibody

References for Anti-Vaccinia Virus antibody (FITC) (ab19970)

This product has been referenced in:
  • Hou W  et al. Oncolytic vaccinia virus demonstrates antiangiogenic effects mediated by targeting of VEGF. Int J Cancer 135:1238-46 (2014). Read more (PubMed: 24474587) »
  • Li Z  et al. A flow cytometry-based immuno-titration assay for rapid and accurate titer determination of modified vaccinia Ankara virus vectors. J Virol Methods 169:87-94 (2010). Read more (PubMed: 20637240) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"