Valinomycin from Streptomyces fulvissimus (ab120852)

Overview

  • Product name
    Valinomycin from Streptomyces fulvissimus
  • Description
    K+ selective ionophore
  • Biological description
    K+ selective ionophore. Natural dodecadepsipeptide. Antitumor, antifungal and antibacterial effects. Apoptosis due to caspase independent mechanisms.
  • Purity
    > 98%

Properties

Applications

Our Abpromise guarantee covers the use of ab120852 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • ab56889 staining mitodusin2 in MEF1 cells treated with valinomycin from Streptomyces fulvissimus (ab120852), by ICC/IF. Decrease in mitofusin2 expression with increased concentration of withaferin valinomycin from Streptomyces fulvissimus, as described in literature.
    The cells were incubated at 37°C for 3h in media containing different concentrations of ab120852 (valinomycin from Streptomyces fulvissimus) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab56889 (10 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

References

This product has been referenced in:
  • Kroten MA  et al. Cereulide and valinomycin, two important natural dodecadepsipeptides with ionophoretic activities. Pol J Microbiol 59:3-10 (2010). Read more (PubMed: 20568524) »
  • Yamada A  et al. Differential permeabilization effects of Ca2+ and valinomycin on the inner and outer mitochondrial membranes as revealed by proteomics analysis of proteins released from mitochondria. Mol Cell Proteomics 8:1265-77 (2009). Read more (PubMed: 19218587) »
  • Duax WL  et al. Molecular structure and mechanisms of action of cyclic and linear ion transport antibiotics. Biopolymers 40:141-55 (1996). Read more (PubMed: 8541445) »

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"

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