Vesicle associated membrane protein 1 synaptobrevin 1 antibody
Vesicle-associated membrane protein 1 antibody
Anti-VAMP1 antibody images
Western blot - VAMP1 antibody (ab3346)
Western blot detection of VAMP1 on rat brain extract using ab3346.
Immunocytochemistry/ Immunofluorescence - VAMP1 antibody (ab3346)This image is courtesy of an anonymous Abreview
ab3346 at a 1/1000 dilution staining mouse adult retina cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with 5% serum prior to incubation with the antibody for 48 hours. Bound antibody was detected using an Alexa Fluor ® 488 conjugated donkey anti rabbit antibody. Dilutions of 1/1000 through to 1/5000 were found to work.
Staining in the outer plexiform layer was fainter than in the ganglion cell layer
ab3346 (2µg/ml) staining VAMP1 in human cerebral cortex using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic and some membrane staining. Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Immunocytochemistry/ Immunofluorescence - Anti-VAMP1 antibody (ab3346)Image from Coller KE et al., PLoS Pathog. 2012 Jan;8(1):e1002466. Epub 2012 Jan 5. Fig S4.; doi:10.1371/journal.ppat.1002466; January 5, 2012, PLoS Pathog 8(1): e1002466.
Immunofluorescence analysis of Huh7.5 cells transfected with GFP-VAMP1, staining VAMP1 with ab3346 at 1/250 dilution. An AlexaFluor®-conjugated IgG (1/1000) was used as the secondary antibody.
References for Anti-VAMP1 antibody (ab3346)
This product has been referenced in:
Zimmermann J et al. Synaptobrevin 1 mediates vesicle priming and evoked release in a subpopulation of hippocampal neurons. J Neurophysiol112:1559-65 (2014).
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