Overview

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab3346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.

Used at a dilution of 1/1000 for 48 hrs incubation on mouse adult retina cells (see Abreview for further information).

IP Use at an assay dependent concentration.
WB Use a concentration of 0.3 µg/ml. Can be blocked with VAMP1 peptide (ab4957). By Western blot, this antibody detects a 15 kDa protein representing VAMP 1 from rat brain whole protein extract.
IHC-P Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • FunctionInvolved in the targeting and/or fusion of transport vesicles to their target membrane.
  • Tissue specificityNervous system, skeletal muscle and adipose tissue.
  • Sequence similaritiesBelongs to the synaptobrevin family.
    Contains 1 v-SNARE coiled-coil homology domain.
  • Cellular localizationMitochondrion outer membrane; Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome and Cytoplasmic vesicle membrane. Cell junction > synapse > synaptosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686H12131 antibody
    • SYB 1 antibody
    • SYB1 antibody
    • Synaptobrevin 1 antibody
    • Synaptobrevin-1 antibody
    • Synaptobrevin1 antibody
    • VAMP 1 antibody
    • VAMP-1 antibody
    • Vamp1 antibody
    • VAMP1_HUMAN antibody
    • Vesicle associated membrane protein 1 antibody
    • Vesicle associated membrane protein 1 synaptobrevin 1 antibody
    • Vesicle-associated membrane protein 1 antibody
    see all

Anti-VAMP1 antibody images

  • Western blot detection of VAMP1 on rat brain extract using ab3346.

  • ab3346 at a 1/1000 dilution staining mouse adult retina cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with 5% serum prior to incubation with the antibody for 48 hours. Bound antibody was detected using an Alexa Fluor ® 488 conjugated donkey anti rabbit antibody. Dilutions of 1/1000 through to 1/5000  were found to work.

    Staining in the outer plexiform layer was fainter than in the ganglion cell layer

    See Abreview

  • ab3346 (2µg/ml) staining VAMP1 in human cerebral cortex using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic and some membrane staining.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • Immunofluorescence analysis of Huh7.5 cells transfected with GFP-VAMP1, staining VAMP1 with ab3346 at 1/250 dilution. An AlexaFluor®-conjugated IgG (1/1000) was used as the secondary antibody.

References for Anti-VAMP1 antibody (ab3346)

This product has been referenced in:
  • Zimmermann J  et al. Synaptobrevin 1 mediates vesicle priming and evoked release in a subpopulation of hippocampal neurons. J Neurophysiol 112:1559-65 (2014). Read more (PubMed: 24944211) »
  • Coller KE  et al. Molecular determinants and dynamics of hepatitis C virus secretion. PLoS Pathog 8:e1002466 (2012). ICC/IF . Read more (PubMed: 22241992) »

See all 7 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Adult retina)
Specification Adult retina
Fixative Paraformaldehyde
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5%
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Submitted Jun 14 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"