Overview

  • Product name
  • Description
    Rabbit polyclonal to VAMP1
  • Tested applications
    Suitable for: IHC-FoFr, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Cow, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Rat VAMP1.

    (Peptide available as ab41454.)

  • Positive control
    • WB: This antibody gave a positive signal in the following tissue lysates: Mouse Brain, Mouse Spinal Cord, Rat Brain and Rat Spinal Cord. IHC: rat brain

Properties

Applications

Our Abpromise guarantee covers the use of ab41324 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr
IHC-P
WB
  • Application notes
    IHC-FoFr: 1/3000.
    WB: Use at a concentration of 1 mg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 13 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Involved in the targeting and/or fusion of transport vesicles to their target membrane.
    • Tissue specificity
      Nervous system, skeletal muscle and adipose tissue.
    • Sequence similarities
      Belongs to the synaptobrevin family.
      Contains 1 v-SNARE coiled-coil homology domain.
    • Cellular localization
      Mitochondrion outer membrane; Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome and Cytoplasmic vesicle membrane. Cell junction > synapse > synaptosome.
    • Information by UniProt
    • Database links
    • Alternative names
      • DKFZp686H12131 antibody
      • SYB 1 antibody
      • SYB1 antibody
      • Synaptobrevin 1 antibody
      • Synaptobrevin-1 antibody
      • Synaptobrevin1 antibody
      • VAMP 1 antibody
      • VAMP-1 antibody
      • Vamp1 antibody
      • VAMP1_HUMAN antibody
      • Vesicle associated membrane protein 1 antibody
      • Vesicle associated membrane protein 1 synaptobrevin 1 antibody
      • Vesicle-associated membrane protein 1 antibody
      see all

    Images

    • All lanes : Anti-VAMP1 antibody (ab41324) at 1 µg/ml

      Lane 1 : Brain (Mouse) Tissue Lysate
      Lane 2 : Brain (Rat) Tissue Lysate - normal tissue
      Lane 3 : Spinal Cord (Mouse) Tissue Lysate
      Lane 4 : Spinal Cord (Rat) Tissue Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size : 13 kDa
      Observed band size : 17 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemistical detection of VAMP1 with ab41324 on rat brain PFA perfusion-fixed frozen sections. Primary antibody ab41324 diluted at 1/3000 and incubated for 18 hours at 20°C in PBS + 0.3 % Triton X100.  Secondary antibody: goat anti-rabbit Alexa Fluor 488 (1/1000)Immunostaining obtained in the rat hippocampus using this antibody diluted 1:1000. Sections come from a perfused-fixed animal. The immunostaining was performed using the `free floating` technique, using direct fluorescence. The antibody stains synapses in many brain areas. The picture shows the staining taken at low power magnification, showing the staining in the hippocampus (top) and medium thalamus (bottom of the picture).

      See Abreview

    References

    ab41324 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Rat Tissue sections (Brain sections)
    Specification
    Brain sections
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    None
    Permeabilization
    No
    Username

    Dr. Sophie Pezet

    Verified customer

    Submitted Feb 18 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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