• Product nameAnti-VAMP1 antibody
    See all VAMP1 primary antibodies
  • Description
    Rabbit polyclonal to VAMP1
  • Tested applicationsSuitable for: IHC-FoFr, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Cow, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Rat VAMP1.

    (Peptide available as ab41454.)

  • Positive control
    • WB: This antibody gave a positive signal in the following tissue lysates: Mouse Brain, Mouse Spinal Cord, Rat Brain and Rat Spinal Cord. IHC: rat brain



Our Abpromise guarantee covers the use of ab41324 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesIHC-FoFr: 1/3000.
    WB: Use at a concentration of 1 mg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 13 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionInvolved in the targeting and/or fusion of transport vesicles to their target membrane.
    • Tissue specificityNervous system, skeletal muscle and adipose tissue.
    • Sequence similaritiesBelongs to the synaptobrevin family.
      Contains 1 v-SNARE coiled-coil homology domain.
    • Cellular localizationMitochondrion outer membrane; Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome and Cytoplasmic vesicle membrane. Cell junction > synapse > synaptosome.
    • Information by UniProt
    • Database links
    • Alternative names
      • DKFZp686H12131 antibody
      • SYB 1 antibody
      • SYB1 antibody
      • Synaptobrevin 1 antibody
      • Synaptobrevin-1 antibody
      • Synaptobrevin1 antibody
      • VAMP 1 antibody
      • VAMP-1 antibody
      • Vamp1 antibody
      • VAMP1_HUMAN antibody
      • Vesicle associated membrane protein 1 antibody
      • Vesicle associated membrane protein 1 synaptobrevin 1 antibody
      • Vesicle-associated membrane protein 1 antibody
      see all

    Anti-VAMP1 antibody images

    • All lanes : Anti-VAMP1 antibody (ab41324) at 1 µg/ml

      Lane 1 : Brain (Mouse) Tissue Lysate
      Lane 2 : Brain (Rat) Tissue Lysate - normal tissue
      Lane 3 : Spinal Cord (Mouse) Tissue Lysate
      Lane 4 : Spinal Cord (Rat) Tissue Lysate

      Lysates/proteins at 10 µg per lane.

      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size : 13 kDa
      Observed band size : 17 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemistical detection of VAMP1 with ab41324 on rat brain PFA perfusion-fixed frozen sections. Primary antibody ab41324 diluted at 1/3000 and incubated for 18 hours at 20°C in PBS + 0.3 % Triton X100.  Secondary antibody: goat anti-rabbit Alexa Fluor 488 (1/1000)Immunostaining obtained in the rat hippocampus using this antibody diluted 1:1000. Sections come from a perfused-fixed animal. The immunostaining was performed using the `free floating` technique, using direct fluorescence. The antibody stains synapses in many brain areas. The picture shows the staining taken at low power magnification, showing the staining in the hippocampus (top) and medium thalamus (bottom of the picture).

      See Abreview

    References for Anti-VAMP1 antibody (ab41324)

    ab41324 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample Rat Tissue sections (Brain sections)
    Specification Brain sections
    Fixative Paraformaldehyde
    Antigen retrieval step None
    Permeabilization No

    Dr. Sophie Pezet

    Verified customer

    Submitted Feb 18 2010