Overview

  • Product name
    Anti-VAMP1 antibody [SP-11]
    See all VAMP1 primary antibodies
  • Description
    Mouse monoclonal [SP-11] to VAMP1
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ELISA, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Tissue, cells or virus corresponding to Human VAMP1. Crude human synaptic immunoprecipitate.

  • Positive control
    • WB: Rat brain tissuelysate. IHC-P: Human brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab98888 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 13 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Target

  • Function
    Involved in the targeting and/or fusion of transport vesicles to their target membrane.
  • Tissue specificity
    Nervous system, skeletal muscle and adipose tissue.
  • Sequence similarities
    Belongs to the synaptobrevin family.
    Contains 1 v-SNARE coiled-coil homology domain.
  • Cellular localization
    Mitochondrion outer membrane; Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome and Cytoplasmic vesicle membrane. Cell junction > synapse > synaptosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686H12131 antibody
    • SYB 1 antibody
    • SYB1 antibody
    • Synaptobrevin 1 antibody
    • Synaptobrevin-1 antibody
    • Synaptobrevin1 antibody
    • VAMP 1 antibody
    • VAMP-1 antibody
    • Vamp1 antibody
    • VAMP1_HUMAN antibody
    • Vesicle associated membrane protein 1 antibody
    • Vesicle associated membrane protein 1 synaptobrevin 1 antibody
    • Vesicle-associated membrane protein 1 antibody
    see all

Images

  • Anti-VAMP1 antibody [SP-11] (ab98888) at 1/1000 dilution + Rat brain lysate at 15 µg

    Secondary
    Sheep Anti-Mouse IgG: HRP

    Predicted band size: 13 kDa



    Block: 1.5% BSA for 30 minutes at RT.

    Incubation with primary antibody for 2 hours at RT.

    Incubation with secondary antibody for 1 hour at RT.

  • Immunohistochemistry analysis of 10% formalin-fixed (12-24 hours at RT) human cerebellum tissue using ab98888 at a 1:1000 dilution for 1 hour at RT.

    Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT.

  • Immunohistochemistry analysis of 10% formalin-fixed (12-24 hours at RT) human brain tissue using ab98888 at a 1:1000 dilution for 1 hour at RT.

    Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT.

References

ab98888 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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