Overview

  • Product nameAnti-VAMP2 antibody [3E5]
    See all VAMP2 primary antibodies
  • Description
    Mouse monoclonal [3E5] to VAMP2
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 1-89 of Human VAMP2, purified from E. coli.

  • Positive control
    • Human Adrenal Human Brain, Cerebellum Human Testis

Properties

Applications

Our Abpromise guarantee covers the use of ab118899 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/3000. Predicted molecular weight: 13 kDa.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionInvolved in the targeting and/or fusion of transport vesicles to their target membrane.
  • Tissue specificityNervous system and skeletal muscle.
  • Sequence similaritiesBelongs to the synaptobrevin family.
    Contains 1 v-SNARE coiled-coil homology domain.
  • Cellular localizationCytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome. Neuronal synaptic vesicles.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ11460 antibody
    • RATVAMPB antibody
    • RATVAMPIR antibody
    • SYB antibody
    • SYB2 antibody
    • Synaptobrevin 2 antibody
    • Synaptobrevin-2 antibody
    • VAMP 2 antibody
    • VAMP-2 antibody
    • Vamp2 antibody
    • VAMP2_HUMAN antibody
    • Vesicle associated membrane protein 2 antibody
    • Vesicle-associated membrane protein 2 (synaptobrevin 2) antibody
    • Vesicle-associated membrane protein 2 antibody
    see all

Anti-VAMP2 antibody [3E5] images

  • Immunohistochemistry analysis (formalin-fixed, paraffin-embedded) of human adrenal stained with ab118899 at 10 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Immunohistochemistry analysis (formalin-fixed, paraffin-embedded) of Human Brain, Cerebellum stained with ab118899 at 10 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Immunohistochemistry analysis (formalin-fixed, paraffin-embedded) of Human Testis stained with ab118899 at 10 ug/ml followed by biotinylated anti-mouse IgG secondary antibody , alkaline phosphatase-streptavidin and chromogen.
  • All lanes : Anti-VAMP2 antibody [3E5] (ab118899) at 1/2000 dilution

    Lane 1 : VAMP1
    Lane 2 : VAMP2
    Lane 3 : Rat brain lysate


    Predicted band size : 13 kDa

References for Anti-VAMP2 antibody [3E5] (ab118899)

ab118899 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"