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Western blot - VAMP2 antibody (ab3347)
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Immunocytochemistry/ Immunofluorescence - VAMP2 antibody (ab3347)
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Product Name
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VAMP2 antibody
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See all VAMP2 antibodies (7)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to VAMP2
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Immunogen
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Synthetic peptide: MSATAATVPPAAPAGEGG-C, corresponding to amino acids 1-18 of Rat VAMP2.
BLAST 'MSATAATVPPAAPAGEGG-C' with  or 
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Reacts with
(species key)
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Ms, Rat
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Tested applications
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ICC/IF, IHC-Fr, IP, WB
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Abreviews
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Application notes
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Recommended dilutions
ICC/IF: 1/1000.
IHC-Fr: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cytoplasmic vesicle; secretory vesicle; synaptic vesicle membrane; Single-pass type IV membrane protein. Cell junction › synapse › synaptosome. Note: Neuronal synaptic vesicles.
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Research areas
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Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Integration of energy metabolism
Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> SNAPs & SNAREs
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Relevance
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VAMP2 (Synaptobrevin 2) is a small integral membrane protein found specifically in synaptic vesicles in neurons. Synaptobrevins/VAMPs, syntaxins, and the 25 kD synaptosomal associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane.
VAMP2 is one of a family of integral membrane proteins of 11-13 kDa with the N terminal region exposed to the cytoplasm and a C terminal transmembrane domain. Two isoforms identified in the mammalian CNS Synaptobrevin 1/VAMP 1 and Synaptobrevin 2/VAMP 2 differ in their distribution within different brain regions. Synaptobrevin/VAMP is highly conserved between vertebrates and invertebrates. It is a major constituent of synaptic vesicles and peptidergic secretory granules in all neurons examined so far. In addition, it is present on secretory granules of neuroendocrine cells. Low levels of synaptobrevin 2/VAMP 2 are present in many other tissues where the protein resides on specialized microvesicles.
In non-neuronal cells, the third isoform, Cellubrevin/VAMP 3, is present where it is localized to an endosomal membrane pool. Synaptobrevin/VAMP is an essential component of the exocytotic fusion machine, related to a larger protein family referred to as v SNAREs. It is the sole target for tetanus and several of the botulinal neurotoxins which cleave the protein at single sites in the C terminal portion of the molecule.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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PBS, 1 mg/ml BSA, 0.05% sodium azide Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this VAMP2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for VAMP2 antibody images, references, products related to ab3347 and other tools.
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VAMP2 antibody images:
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 Western blot - VAMP2 antibody (ab3347)
Predicted band size : 19 kDa
Western blot of VAMP-2 on rat brain extract using ab3347.
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Immunocytochemistry/ Immunofluorescence - VAMP2 antibody (ab3347)
VAMP2 antibody (ab3347) used in Immunohistochemistry (rat frozen sections). Sections were fixed with paraformaldehyde. Primary Antibody ab3347 used at 1/1000 incubated for 18 hours at 20°C in PBS-T 0.3%. Secondary Antibody used anti-IgG Rabbit Conjugation Alexa Fluor® 488 Dilution 1/1000. Karine Thibault, Laboratoire de Neurobiologie, Paris
See Abreview
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References for VAMP2 antibody (ab3347)
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This product has been used in: (two most recent references)
Amin ND et al. Cyclin-dependent kinase 5 phosphorylation of human septin SEPT5 (hCDCrel-1) modulates exocytosis. J Neurosci 28:3631-43 (2008). PubMed: 18385322
Chen XW et al. DTNBP1, a schizophrenia susceptibility gene, affects kinetics of transmitter release. J Cell Biol 181:791-801 (2008). WB; Mouse. PubMed: 18504299
See all 4 publication references for this product.
If you publish research using ab3347 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to VAMP2
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