Overview

  • Product nameAnti-VAMP2 antibody
    See all VAMP2 primary antibodies
  • Description
    Rabbit polyclonal to VAMP2
  • Tested applicationsSuitable for: WB, IP, IHC-Fr, ICC/IF, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
    Predicted to work with: Rhesus monkey
  • Immunogen

    Synthetic peptide corresponding to Rat VAMP2 aa 1-18.
    Sequence:

    MSATAATVPPAAPAGEGG


    (Peptide available as ab4958)

Properties

Applications

Our Abpromise guarantee covers the use of ab3347 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).Can be blocked with VAMP2 peptide (ab4958).
IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/1000.
IHC-FoFr Use at an assay dependent concentration. PubMed: 23056393

Target

  • FunctionInvolved in the targeting and/or fusion of transport vesicles to their target membrane.
  • Tissue specificityNervous system and skeletal muscle.
  • Sequence similaritiesBelongs to the synaptobrevin family.
    Contains 1 v-SNARE coiled-coil homology domain.
  • Cellular localizationCytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome. Neuronal synaptic vesicles.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ11460 antibody
    • RATVAMPB antibody
    • RATVAMPIR antibody
    • SYB antibody
    • SYB2 antibody
    • Synaptobrevin 2 antibody
    • Synaptobrevin-2 antibody
    • VAMP 2 antibody
    • VAMP-2 antibody
    • Vamp2 antibody
    • VAMP2_HUMAN antibody
    • Vesicle associated membrane protein 2 antibody
    • Vesicle-associated membrane protein 2 (synaptobrevin 2) antibody
    • Vesicle-associated membrane protein 2 antibody
    see all

Anti-VAMP2 antibody images



  • Predicted band size : 19 kDa

    Western blot detection of VAMP2 on rat brain extract using ab3347.

  • VAMP2 antibody (ab3347) used in Immunohistochemistry (rat frozen sections). Sections were fixed with paraformaldehyde. Primary Antibody ab3347 used at 1/1000 incubated for 18 hours at 20°C in PBS-T 0.3%.  Secondary Antibody used anti-IgG Rabbit Conjugation Alexa Fluor® 488 Dilution 1/1000.

    See Abreview

  • Immunohistochemical analysis of rat spinal cord dorsal horn tissue, staining VAMP2 with ab3347 at 1/200 dilution. Fluorescent-conjugated anti-rabbit IgG (1/200) was used as the secondary antibody.

References for Anti-VAMP2 antibody (ab3347)

This product has been referenced in:
  • Gordon SL  et al. A Fine Balance of Synaptophysin Levels Underlies Efficient Retrieval of Synaptobrevin II to Synaptic Vesicles. PLoS One 11:e0149457 (2016). Mouse . Read more (PubMed: 26871701) »
  • Kosiorek M  et al. Calcineurin/NFAT signaling represses genes Vamp1 and Vamp2 via PMCA-dependent mechanism during dopamine secretion by Pheochromocytoma cells. PLoS One 9:e92176 (2014). ICC/IF ; Rat . Read more (PubMed: 24667359) »

See all 14 Publications for this product

Product Wall

Application Western blot
Loading amount 2 µg
Gel Running Conditions Non-reduced Denaturing
Sample Rat Tissue lysate - whole (hippocampus tissue lysate)
Specification hippocampus tissue lysate
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 27°C
Username

Abcam user community

Verified customer

Submitted Jul 07 2014

Thank you for your reply and the additional information.

Please let me know your order or PO number. If the antibody was purchased within the last 6 months, our Abpromise guarantee applies and I'd be happy to offer you either a free of charge ...

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Thank you for contacting us.
I am sorry to hear that the antibody is not working as expected.

I have copied the WB protocol below.
Also,
I have a few more questions in order to understand the issue better:
1) What is the exact...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Permeabilization No
Username

Dr. Karine Thibault

Verified customer

Submitted Apr 14 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (hippocampal neuron)
Specification hippocampal neuron
Fixative Paraformaldehyde
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 2%
Username

Dr. Chanchoo Yap

Verified customer

Submitted Nov 27 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"