Anti-VAMP2 antibody (ab3347)
Key features and details
- Rabbit polyclonal to VAMP2
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-VAMP2 antibody
See all VAMP2 primary antibodies -
Description
Rabbit polyclonal to VAMP2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Rhesus monkey -
Immunogen
Synthetic peptide corresponding to Rat VAMP2 aa 1-18.
Sequence:MSATAATVPPAAPAGEGG
(Peptide available asab4958) -
Positive control
- WB: Mouse brain and Rat brain lysates
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituents: PBS, 0.1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3347 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
Use at an assay dependent concentration. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa). |
Target
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Function
Involved in the targeting and/or fusion of transport vesicles to their target membrane. -
Tissue specificity
Nervous system and skeletal muscle. -
Sequence similarities
Belongs to the synaptobrevin family.
Contains 1 v-SNARE coiled-coil homology domain. -
Cellular localization
Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome. Neuronal synaptic vesicles. - Information by UniProt
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Database links
- Entrez Gene: 22318 Mouse
- Entrez Gene: 24803 Rat
- SwissProt: P63044 Mouse
- SwissProt: P63045 Rat
- Unigene: 28643 Mouse
- Unigene: 12939 Rat
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Alternative names
- FLJ11460 antibody
- RATVAMPB antibody
- RATVAMPIR antibody
see all
Images
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All lanes : Anti-VAMP2 antibody (ab3347) at 1 µg/ml
Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lane 3 : Mouse lung lysate
Lane 4 : Rat lung lysate
Lane 5 : Rat liver lysate
Lane 6 : Rat kidney lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 1/4000 dilution
Predicted band size: 19 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (46)
ab3347 has been referenced in 46 publications.
- Lu Z et al. Base-edited cynomolgus monkeys mimic core symptoms of STXBP1 encephalopathy. Mol Ther 30:2163-2175 (2022). PubMed: 35283272
- Chen Y et al. Synaptotagmin-1 interacts with PI(4,5)P2 to initiate synaptic vesicle docking in hippocampal neurons. Cell Rep 34:108842 (2021). PubMed: 33730593
- Wilhelmi I et al. The ARFRP1-dependent Golgi scaffolding protein GOPC is required for insulin secretion from pancreatic ß-cells. Mol Metab 45:101151 (2021). PubMed: 33359402
- Ivanova D et al. Control of synaptic vesicle release probability via VAMP4 targeting to endolysosomes. Sci Adv 7:N/A (2021). PubMed: 33931449
- Rong X et al. Enalapril attenuates endoplasmic reticulum stress and mitochondrial injury induced by myocardial infarction via activation of the TAK1/NFAT pathway in mice. Exp Ther Med 19:972-980 (2020). PubMed: 32010259