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Mouse monoclonal [clone 3E5] to VAMP2
This antibody recognizes specifically VAMP2, but it also shows a low affinity to VAMP1.
HumanPredicted to work with:
Dog, Chimpanzee, Rhesus monkey
Recombinant fragment: MSATAATAPP AAPAGEGGPP APPPNLTSNR RLQQTQAQVD EVVDIMRVNV DKVLERDQKL SELDDRADAL QAGASQFETS AAKLKRKYW, corresponding to amino acids 1-89 of Human VAMP2
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4
Concentration information loading...
Protein G purified
Light chain type
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/2000 - 1/3000. Predicted molecular weight: 13 kDa.
||Use at an assay dependent dilution.
Involved in the targeting and/or fusion of transport vesicles to their target membrane.
Nervous system and skeletal muscle.
Belongs to the synaptobrevin family.
Contains 1 v-SNARE coiled-coil homology domain.
Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome. Neuronal synaptic vesicles.
Information by UniProt
- FLJ11460 antibody
- RATVAMPB antibody
- RATVAMPIR antibody
Western blot - VAMP2 antibody [clone 3E5] (ab53407)
All lanes :
Anti-VAMP2 antibody [clone 3E5] (ab53407) at 1/2000 dilutionLane 1 :
Recombinant VAMP1Lane 2 :
Recombinant VAMP2Lane 3 :
Rat brain extracts
Lysates/proteins at 20 µg per lane.Secondary
HRP-conjugated goat anti-mouse
Developed using the ECL techniquePredicted band size :
13 kDaObserved band size :
13,15,17 kDa (why is the actual band size different from the predicted?
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"