Overview

  • Product nameAnti-VAMP2 antibody [clone 3E5]
    See all VAMP2 primary antibodies
  • Description
    Mouse monoclonal [clone 3E5] to VAMP2
  • SpecificityThis antibody recognizes specifically VAMP2, but it also shows a low affinity to VAMP1.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Dog, Chimpanzee, Rhesus monkey
  • Immunogen

    Recombinant fragment: MSATAATAPP AAPAGEGGPP APPPNLTSNR RLQQTQAQVD EVVDIMRVNV DKVLERDQKL SELDDRADAL QAGASQFETS AAKLKRKYW, corresponding to amino acids 1-89 of Human VAMP2

  • Positive control
    • Rat brain extracts

Properties

Applications

Our Abpromise guarantee covers the use of ab53407 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/3000. Predicted molecular weight: 13 kDa.
ELISA Use at an assay dependent dilution.

Target

  • FunctionInvolved in the targeting and/or fusion of transport vesicles to their target membrane.
  • Tissue specificityNervous system and skeletal muscle.
  • Sequence similaritiesBelongs to the synaptobrevin family.
    Contains 1 v-SNARE coiled-coil homology domain.
  • Cellular localizationCytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome. Neuronal synaptic vesicles.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ11460 antibody
    • RATVAMPB antibody
    • RATVAMPIR antibody
    • SYB antibody
    • SYB2 antibody
    • Synaptobrevin 2 antibody
    • Synaptobrevin-2 antibody
    • VAMP 2 antibody
    • VAMP-2 antibody
    • Vamp2 antibody
    • VAMP2_HUMAN antibody
    • Vesicle associated membrane protein 2 antibody
    • Vesicle-associated membrane protein 2 (synaptobrevin 2) antibody
    • Vesicle-associated membrane protein 2 antibody
    see all

Anti-VAMP2 antibody [clone 3E5] images

  • All lanes : Anti-VAMP2 antibody [clone 3E5] (ab53407) at 1/2000 dilution

    Lane 1 : Recombinant VAMP1
    Lane 2 : Recombinant VAMP2
    Lane 3 : Rat brain extracts

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP-conjugated goat anti-mouse
    Developed using the ECL technique

    Predicted band size : 13 kDa
    Observed band size : 13,15,17 kDa (why is the actual band size different from the predicted?)

References for Anti-VAMP2 antibody [clone 3E5] (ab53407)

ab53407 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"