Overview

  • Product nameAnti-VAMP4 antibody
    See all VAMP4 primary antibodies
  • Description
    Rabbit polyclonal to VAMP4
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    KLH conjugated synthetic peptide between internal sequence amino acids 23 - 53 of Human VAMP4 (NP_001172056.1, NP_003753.2).

  • Positive control
    • WB: K562 cell line lysates; Mouse liver tissue lysates IHC-P: Human hepatocarcinoma

Properties

Applications

Our Abpromise guarantee covers the use of ab108051 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 16 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionInvolved in the pathway that functions to remove an inhibitor (probably synaptotagmin-4) of calcium-triggered exocytosis during the maturation of secretory granules. May be a marker for this sorting pathway that is critical for remodeling the secretory response of granule.
  • Sequence similaritiesBelongs to the synaptobrevin family.
    Contains 1 v-SNARE coiled-coil homology domain.
  • Cellular localizationGolgi apparatus > trans-Golgi network membrane. Associated with trans Golgi network (TGN) and newly formed immature secretory granules (ISG). Not found on the mature secretory organelles.
  • Information by UniProt
  • Database links
  • Alternative names
    • VAMP 24 antibody
    • VAMP 4 antibody
    • VAMP-4 antibody
    • VAMP24 antibody
    • VAMP4 antibody
    • VAMP4 protein antibody
    • VAMP4_HUMAN antibody
    • Vesicle associated membrane protein 4 antibody
    • Vesicle associated membrane protein 4 isoform CRA a antibody
    • Vesicle associated membrane protein 4 isoform CRA b antibody
    • Vesicle-associated membrane protein 4 antibody
    see all

Anti-VAMP4 antibody images

  • Anti-VAMP4 antibody (ab108051) at 1/100 dilution + K562 cell line lysates at 35 µg

    Predicted band size : 16 kDa
  • Anti-VAMP4 antibody (ab108051) at 1/100 dilution + Mouse liver tissue lysates at 35 µg

    Predicted band size : 16 kDa
  • ab108051, at a 1/50 dilution, staining VAMP4 in formalin fixed, paraffin embedded Human hepatocarcinoma by Immunohistochemistry, followed by peroxidase conjugation of the secondary antibody and DAB staining.

References for Anti-VAMP4 antibody (ab108051)

ab108051 has not yet been referenced specifically in any publications.

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