Overview

  • Product nameAnti-VAMP4 antibody
    See all VAMP4 primary antibodies
  • Description
    Rabbit polyclonal to VAMP4
  • Tested applicationsSuitable for: ICC/IF, WB, Electron Microscopy, IHC-Fr, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Other Immunogen Type corresponding to Rat VAMP4. Recombinant rat VAMP4 protein.

  • Positive control
    • WB: PC3 cell extract ICC: CV-1 cells

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferConstituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Primary antibody notesThe vesicle associated membrane proteins (VAMP) or synaptobrevins are calcium binding proteins specific to eukaryotes. VAMPs, along with synaptosomal associated protein of 25 kDa (SNAP 25) and syntaxin, form the core complex of soluble NSF attachment protein receptor (SNARE) proteins that interact with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and alpha-SNAP. These membrane associated proteins play a key role in the regulation of vesicle membrane fusion with the plasma membrane. The Clostridium tetani neurotoxin is a metalloprotease with specificity for VAMP. In Alzheimer’s disease, VAMP levels of all isoforms appear to be significantly lowered.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3348 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 16 kDa).
Electron Microscopy Use at an assay dependent concentration. PubMed: 23770993
IHC-Fr Use at an assay dependent concentration. PubMed: 21116650
IP Use at an assay dependent concentration. PubMed: 17922004
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionInvolved in the pathway that functions to remove an inhibitor (probably synaptotagmin-4) of calcium-triggered exocytosis during the maturation of secretory granules. May be a marker for this sorting pathway that is critical for remodeling the secretory response of granule.
  • Sequence similaritiesBelongs to the synaptobrevin family.
    Contains 1 v-SNARE coiled-coil homology domain.
  • Cellular localizationGolgi apparatus > trans-Golgi network membrane. Associated with trans Golgi network (TGN) and newly formed immature secretory granules (ISG). Not found on the mature secretory organelles.
  • Information by UniProt
  • Database links
  • Alternative names
    • VAMP 24 antibody
    • VAMP 4 antibody
    • VAMP-4 antibody
    • VAMP24 antibody
    • VAMP4 antibody
    • VAMP4 protein antibody
    • VAMP4_HUMAN antibody
    • Vesicle associated membrane protein 4 antibody
    • Vesicle associated membrane protein 4 isoform CRA a antibody
    • Vesicle associated membrane protein 4 isoform CRA b antibody
    • Vesicle-associated membrane protein 4 antibody
    see all

Anti-VAMP4 antibody images

  • Anti-VAMP4 antibody (ab3348) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 16 kDa
    Observed band size : 16 kDa
    Additional bands at : 36 kDa,49 kDa,75 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized human cervical carcinoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1/200 with a rabbit polyclonal antibody recognizing VAMP4 (ab3348) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  • Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized human heart tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1/200 with a rabbit polyclonal antibody recognizing VAMP4 (ab3348) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  • Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized human tonsil tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1/200 with a rabbit polyclonal antibody recognizing VAMP4 (ab3348) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

References for Anti-VAMP4 antibody (ab3348)

This product has been referenced in:
  • Amrutkar M  et al. Protein kinase STK25 regulates hepatic lipid partitioning and progression of liver steatosis and NASH. FASEB J 29:1564-76 (2015). WB ; Mouse . Read more (PubMed: 25609431) »
  • Messenger SW  et al. Vesicle associated membrane protein 8 (VAMP8)-mediated zymogen granule exocytosis is dependent on endosomal trafficking via the constitutive-like secretory pathway. J Biol Chem 289:28040-53 (2014). Read more (PubMed: 25138214) »

See all 11 Publications for this product

Product Wall

Thank you for your enquiry regarding ab3348. The positive control for WB is PC3 cell extract which you can purchase from Abcam via our Japanese distributor Cosmobio, catalogue number ab3954. http://www.abcam.com/index.html?datasheet=3954 I wou...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"