The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. PubMed: 24550300
Use a concentration of 1 µg/ml. Predicted molecular weight: 11 kDa.
FunctionInvolved in the targeting and/or fusion of transport vesicles to their target membrane. Involved for dense-granule secretion in platelets. Plays a role in regulated enzyme secretion in pancreatic acinar cells. Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells. Involved in the homotypic fusion of early and late endosomes.
Sequence similaritiesBelongs to the synaptobrevin family. Contains 1 v-SNARE coiled-coil homology domain.
Anti-VAMP8 antibody (ab89158) at 1 µg/ml + A431 whole cell lysate (ab7909) at 25 µg
Predicted band size : 11 kDa
Western blot - VAMP8 antibody (ab89158)
Anti-VAMP8 antibody (ab89158) at 1 µg/ml + Transfected 293T lysate at 25 µg
Predicted band size : 11 kDa Observed band size : 11 kDa
References for Anti-VAMP8 antibody (ab89158)
This product has been referenced in:
Pirooz SD et al. UVRAG is required for virus entry through combinatorial interaction with the class C-Vps complex and SNAREs. Proc Natl Acad Sci U S A111:2716-21 (2014).
Read more (PubMed: 24550300) »
Messenger SW et al. Vesicle associated membrane protein 8 (VAMP8)-mediated zymogen granule exocytosis is dependent on endosomal trafficking via the constitutive-like secretory pathway. J Biol Chem289:28040-53 (2014).
Read more (PubMed: 25138214) »