Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human VAMP8 (N terminal). The exact sequence is proprietary.
WB: 293T, HEK293, HeLa, RAW264.7, NIH/3T3 and PC-12 cell lysates and mouse kidney tissue lysate.
IHC-P: Human brain and kidney tissue.
ICC/IF: PC-12 cells.
Flow Cyt: HeLa cells.
IP: HEK293 cell lysate.
This product is a recombinant rabbit monoclonal antibody.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
1/100 - 1/250.
Involved in the targeting and/or fusion of transport vesicles to their target membrane. Involved for dense-granule secretion in platelets. Plays a role in regulated enzyme secretion in pancreatic acinar cells. Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells. Involved in the homotypic fusion of early and late endosomes.
Belongs to the synaptobrevin family. Contains 1 v-SNARE coiled-coil homology domain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling VAMP8 with purified ab76021 at a dilution of 1/250. Heat mediated antigen retrieval was performed using EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunocytochemistry/Immunofluorescence analysis of PC-12 cells labelling VAMP8 with purified ab76021 at a dilution of 1/100. Cells were fixed with 100% methanol and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.
Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).
Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).
Flow Cytometry analysis of HeLa cells labelling VAMP8 with purified ab76021 at a dilution of 1/150 (red). Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue labelling VAMP8 with unpurified ab76021 at a dilution of 1/100. A HRP/AP polymerized secondary antibody was used.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP8 antibody [EP2629Y] (ab76021)Image from Kamoi M et al., PLoS One. 2012;7(9):e43688. doi: 10.1371/journal.pone.0043688. Epub 2012 Sep 4. Fig 4.; doi:10.1371/journal.pone.0043688; September 4, 2012, PLoS ONE 7(9): e43688.
Immunohistochemical analysis of Human lacrimal gland tissue staining VAMP8 with unpurified ab76021.
Antigen retrieval was performed using antigen retrieval solution in a microwave. Sections were blocked with 10 goat serum for 30 minutes and incubated with primary antibody (1/100) overnight at 4°C. Staining was detected using DAB.
References for Anti-VAMP8 antibody [EP2629Y] (ab76021)
This product has been referenced in:
Xie X et al. Deep vein thrombosis is accurately predicted by comprehensive analysis of the levels of microRNA-96 and plasma D-dimer. Exp Ther Med12:1896-1900 (2016).
Read more (PubMed: 27588107) »
McLelland GL et al. Syntaxin-17 delivers PINK1/parkin-dependent mitochondrial vesicles to the endolysosomal system. J Cell Biol214:275-91 (2016).
Read more (PubMed: 27458136) »