VAMP8 overexpression 293T lysate (whole cell) (ab94103)

Overview

  • Product name
    VAMP8 overexpression 293T lysate (whole cell)
  • Description
    VAMP8 overexpression lysate
  • General notes
    ab94103 is a 293T cell transfected lysate in which Human VAMP8 has been transiently over-expressed using a pCMV-VAMP8 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applications
    Suitable for: WBmore details

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 6.80
    Constituent: 100% 1x Sample Buffer
  • Concentration information loading...
  • Purity
    Whole Cell Lysate
  • Research areas
  • Background
    Function: Involved in the targeting and/or fusion of transport vesicles to their target membrane. Involved for dense-granule secretion in platelets. Plays a role in regulated enzyme secretion in pancreatic acinar cells. Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells. Involved in the homotypic fusion of early and late endosomes. Tissue specificity: Platelets. Similarity: Belongs to the synaptobrevin family. Contains 1 v-SNARE coiled-coil homology domain.

Associated products

Applications

Our Abpromise guarantee covers the use of ab94103 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

Images

  • ab94103 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-VAMP8 antibody (ab89158) at 1/500 dilution

    Lane 1 : VAMP8 overexpression 293T lysate (whole cell) (ab94103)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References

ab94103 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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