The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: using 1ug of vancomycin-BSA coated to the plate, the end-point was observed at an antibody dilution of >1:64000.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceUntil recently, Vancomycin was one of the most powerful antibiotics that no bacterial cell had resistance to. Vancomycin is a very successful glycopeptide antibiotic, attacking the D alanyl D alanine component of the cell wall. By binding to the D alanyl D alanine component, Vancomycin is able to interrupt the normal cell wall formation. However, recently cells have achieved resistance to vancomycin. The reason that the resistance is so effective is that these cells have modified the D alanyl D alanine components of the cell wall into D alanyl D lactate components. Although this may sound easy, the actual process involves a series of five or more genes.
Anti-Vancomycin antibody images
ELISA - Anti-Vancomycin antibody (ab19968)Image courtesy of Dr Julie Richards by Abreview.
ab19968 used at a 1/1000 dilution in ELISA. ab97064 Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) used as the secondary at a 1/1000 dilution. Blocking step performed using 2.5% milk for 30 minutes at 20°C.