Anti-Vancomycin antibody (ab19968)

Overview

  • Product name
  • Description
    Rabbit polyclonal to Vancomycin
  • Specificity
    Vancomycin
  • Tested applications
    Suitable for: ELISAmore details
  • Immunogen

    Chemical/ Small Molecule (Vancomycin conjugated to KLH).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    Ammonium Sulphate fractionation.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab19968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
  • Application notes
    ELISA: using 1ug of vancomycin-BSA coated to the plate, the end-point was observed at an antibody dilution of >1:64000.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Until recently, Vancomycin was one of the most powerful antibiotics that no bacterial cell had resistance to. Vancomycin is a very successful glycopeptide antibiotic, attacking the D alanyl D alanine component of the cell wall. By binding to the D alanyl D alanine component, Vancomycin is able to interrupt the normal cell wall formation. However, recently cells have achieved resistance to vancomycin. The reason that the resistance is so effective is that these cells have modified the D alanyl D alanine components of the cell wall into D alanyl D lactate components. Although this may sound easy, the actual process involves a series of five or more genes.

    Anti-Vancomycin antibody images

    • ab19968 used at a 1/1000 dilution in ELISA.
      ab97064 Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) used as the secondary at a 1/1000 dilution.

      Blocking step performed using 2.5% milk for 30 minutes at 20°C.

      See Abreview

    References for Anti-Vancomycin antibody (ab19968)

    ab19968 has not yet been referenced specifically in any publications.

    Product Wall

    Merci de votre intérêt pour les anticorps Abcam.

    Veuillez trouver ci-joint le protocole FPIArecommandé par le laboratoirequi produit l'anticorps ab30851.

    Nous ignorons si la référence ab19968 foncti...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    ELISA
    Sample
    Human Serum (Drug)
    Specification
    Drug
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 20°C
    Type
    Direct
    Username

    Dr. Julie Richards

    Verified customer

    Submitted Jan 17 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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