The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution. Preliminary experiments gave an approx 55kDa band in Human Adipose and Lung lysates after 0.01µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 84.6kDa according to NP_003725.1. The 55kDa band was successfully blocked by incubation with the immunizing peptide.
Use a concentration of 3 - 5 µg/ml. In paraffin embedded Human Liver shows membranous staining in sinusoid lining cells surrounding hepatocytes.
FunctionCell adhesion protein that participates in lymphocyte recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin-independent fashion. Has a monoamine oxidase activity. May play a role in adipogenesis.
Tissue specificityStrongly expressed on the high endothelial venules of peripheral lymph nodes and on hepatic endothelia. Also highly expressed in appendix, lung and small intestine. Expressed also in adipose tissue, in bone marrow, colon, heart, kidney, ovary, pancreas, placenta, prostate, skeletal muscle, spleen and testis.
Sequence similaritiesBelongs to the copper/topaquinone oxidase family.
Post-translational modificationsTopaquinone (TPQ) is generated by copper-dependent autoxidation of a specific tyrosyl residue. N- and O-glycosylated.
ab59037 at 1 µg/ml staining VAP1 in Human liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6 was performed. Antibody detected with AP-staining.
References for Anti-VAP1 antibody (ab59037)
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