Overview

  • Product nameAnti-VAPA antibody
    See all VAPA primary antibodies
  • Description
    Mouse monoclonal to VAPA
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length Human VAPA protein (AAH02992) with tag.

  • Positive control
    • VAPA transfected 293T cell lysate. Recombinant tagged VAPA protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab77002 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 27 kDa.
ELISA Use at an assay dependent dilution.

Target

  • FunctionMay play a role in vesicle trafficking.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the VAMP-associated protein (VAP) (TC 9.B.17) family.
    Contains 1 MSP domain.
  • Cellular localizationEndoplasmic reticulum membrane. Present in the plasma membrane and in intracellular vesicles, together with SNARE proteins. May also associate with the cytoskeleton. Colocalizes with OCLN at the tight junction in polarized epithelial cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • 33 kDa Vamp-associated protein antibody
    • hVAP 33 antibody
    • MGC3745 antibody
    • OTTHUMP00000162362 antibody
    • OTTHUMP00000162363 antibody
    • VAMP (vesicle-associated membrane protein) associated protein A, 33kDa antibody
    • VAMP A antibody
    • VAMP associated protein A antibody
    • VAMP-A antibody
    • VAMP-associated protein A antibody
    • VAP 33 antibody
    • VAP A antibody
    • VAP-33 antibody
    • VAP-A antibody
    • VAP33 antibody
    • Vapa antibody
    • VAPA_HUMAN antibody
    • Vesicle associated membrane protein associated protein A antibody
    • Vesicle-associated membrane protein-associated protein A antibody
    see all

Anti-VAPA antibody images

  • Anti-VAPA antibody (ab77002) at 1 µg/ml + Recombinant tagged VAPA protein at 0.1 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 27 kDa
    Observed band size : 53 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-VAPA antibody (ab77002) at 1 µg/ml

    Lane 1 : VAPA transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 27 kDa
    Observed band size : 27 kDa

References for Anti-VAPA antibody (ab77002)

ab77002 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"