Anti-VAPB antibody (ab103638)
Key features and details
- Rabbit polyclonal to VAPB
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-VAPB antibody
See all VAPB primary antibodies -
Description
Rabbit polyclonal to VAPB -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant full length protein within Human VAPB. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- Mouse lung tissue lysate; HepG2 cell lysate; VAPB transfected 293T cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab103638 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/500 - 1/1000. Predicted molecular weight: 27 kDa.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 27 kDa. |
Target
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Function
Participates in the endoplasmic reticulum unfolded protein response (UPR) by inducing ERN1/IRE1 activity. Involved in cellular calcium homeostasis regulation. -
Tissue specificity
Ubiquitous. Isoform 1 predominates. -
Involvement in disease
Amyotrophic lateral sclerosis 8
Spinal muscular atrophy, proximal, adult, autosomal dominant -
Sequence similarities
Belongs to the VAMP-associated protein (VAP) (TC 9.B.17) family.
Contains 1 MSP domain. -
Cellular localization
Endoplasmic reticulum membrane. Present in mitochondria-associated membranes that are endoplasmic reticulum membrane regions closely apposed to the outer mitochondrial membrane. - Information by UniProt
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Database links
- Entrez Gene: 9217 Human
- Entrez Gene: 56491 Mouse
- Omim: 605704 Human
- SwissProt: O95292 Human
- SwissProt: Q9QY76 Mouse
- Unigene: 182625 Human
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Alternative names
- ALS 8 antibody
- ALS8 antibody
- D2Abb2e antibody
see all
Images
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Anti-VAPB antibody (ab103638) at 1/500 dilution + Mouse lung tissue lysate at 50 µg
Predicted band size: 27 kDa -
Anti-VAPB antibody (ab103638) at 1/500 dilution + HepG2 cell lysate at 50 µg
Predicted band size: 27 kDa -
All lanes : Anti-VAPB antibody (ab103638) at 1/500 dilution
Lane 1 : VAPB transfected 293T cell line lysate
Lane 2 : Non-transfected 293T cell line lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 27 kDa
Datasheets and documents
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Datasheet download
References (2)
ab103638 has been referenced in 2 publications.
- Papadopoulou AA et al. Signal Peptide Peptidase-Like 2c (SPPL2c) impairs vesicular transport and cleavage of SNARE proteins. EMBO Rep 20:N/A (2019). PubMed: 30733281
- Papadopoulou AA et al. Signal peptide peptidase-like 2c impairs vesicular transport and cleaves SNARE proteins. EMBO Rep 20:e46451 (2019). PubMed: 32048434