• Product name
  • Description
    Rabbit polyclonal to VARP
  • Tested applications
    Suitable for: ICC/IF, WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region between amino acids 1000-1050 of Human VARP (NP_115515.2).

  • Positive control
    • HeLa and 293T whole cell lysates IF/ICC: HeLa cell line.


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab108216 was affinity purified using an epitope specific to VARP immobilized on solid support.
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab108216 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/2000 - 1/10000. Predicted molecular weight: 117 kDa.
IP Use at 2-5 µg/mg of lysate.


  • Function
    May be a Rab21 guanine exchange factor and regulate endosome dynamics.
  • Sequence similarities
    Contains 11 ANK repeats.
    Contains 1 VPS9 domain.
  • Cellular localization
    Cytoplasm. Mainly localized to early endosomes in the perinuclear region.
  • Information by UniProt
  • Database links
  • Alternative names
    • AA408090 antibody
    • Ankrd27 antibody
    • Ankyrin repeat domain 27 (VPS9 domain) antibody
    • Ankyrin repeat domain-containing protein 27 antibody
    • ANR27_HUMAN antibody
    • BC016493 antibody
    • D330003H11Rik antibody
    • DKFZp434L0718 antibody
    • FLJ00040 antibody
    • MGC25907 antibody
    • PP12899 antibody
    • VPS9 ankyrin repeat protein antibody
    • VPS9 domain-containing protein antibody
    see all

Anti-VARP antibody images

  • ab108216 stained HeLa cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab108216 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • All lanes : Anti-VARP antibody (ab108216) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 117 kDa

    Exposure time : 3 minutes
  • Detection of VARP by Western Blot of Immunprecipitate.
    Lane 1: ab108216 at 1µg/ml staining VARP in HeLa whole cell lysate immunoprecipitated using ab108216 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Lane 2: Control IgG
    Detection: Chemiluminescence with exposure time of 30 seconds.

References for Anti-VARP antibody (ab108216)

This product has been referenced in:
  • McGough IJ  et al. Identification of molecular heterogeneity in SNX27-retromer-mediated endosome-to-plasma-membrane recycling. J Cell Sci 127:4940-53 (2014). Read more (PubMed: 25278552) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab108216.
Please use the links above to contact us or submit feedback about this product.


Sign up