Overview

  • Product nameAnti-VASH1 antibody
    See all VASH1 primary antibodies
  • Description
    Mouse polyclonal to VASH1
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length VASH1 protein (Human)

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab67423 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution. PubMed: 20708159
WB 1/500 - 1/1000. Detects a band of approximately 24 kDa (predicted molecular weight: 22 kDa).

Target

  • FunctionAngiogenesis inhibitor. Inhibits migration, proliferation and network formation by endothelial cells as well as angiogenesis. This inhibitory effect is selective to endothelial cells as it does not affect the migration of smooth muscle cells or fibroblasts. Does not affect the proliferation of cancer cells in vitro, but inhibits tumor growth and tumor angiogenesis. Acts in an autocrine manner. Inhibits artery neointimal formation and macrophage infiltration. Exhibits heparin-binding activity.
  • Tissue specificityPreferentially expressed in endothelial cells. Highly expressed in fetal organs. Expressed in brain and placenta, and at lower level in heart and kidney. Highly detected in microvessels endothelial cells of atherosclerotic lesions.
  • Sequence similaritiesBelongs to the vasohibin family.
  • Post-translational
    modifications
    2 major forms (42 and 36 kDa) and 2 minors (32 and 27 kDa) may be processed by proteolytic cleavage. The largest form (42 kDa) seems to be secreted and the other major form (63 kDa) seems to accumulate within the cells or pericellular milieu. Polypeptide consisting of Met-77 to Arg-318 may correspond to the 27 kDa form and that consisting of Met-77 to Val-365 may correspond to the 36 kDa form.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • KIAA1036 antibody
    • VASH antibody
    • VASH1 antibody
    • VASH1_HUMAN antibody
    • Vasohibin 1 antibody
    • Vasohibin-1 antibody
    see all

Anti-VASH1 antibody images

  • All lanes : Anti-VASH1 antibody (ab67423) at 1/500 dilution

    Lane 1 : VASH1 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg/ml per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 22 kDa
    Observed band size : 24 kDa (why is the actual band size different from the predicted?)

References for Anti-VASH1 antibody (ab67423)

This product has been referenced in:

See 1 Publication for this product

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