Anti-Vasopressin antibody (ab68669)

Overview

  • Product nameAnti-Vasopressin antibody
    See all Vasopressin primary antibodies
  • Description
    Rabbit polyclonal to Vasopressin
  • Tested applicationsSuitable for: IHC-FoFr, IHC-P, ELISAmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Sheep, Human
    Predicted to work with: Mouse, Rat, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Vasopressin.

    (Peptide available as ab68668.)

  • Positive control
    • This antibody gave a positive signal in IHC when used to probe human hypothalamus tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab68669 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr 1/100.
IHC-P Use a concentration of 1 - 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ELISA Use a concentration of 0.002 µg/ml.
  • Application notesIs unsuitable for WB.
  • Target

    • RelevanceVasopressin, also known as arginine vasopressin (AVP) or antidiuretic hormone (ADH), is a posterior pituitary hormone that is synthesised in the hypothalamus. Vasopressin is synthesised as a precursor protein that consists of arginine vasopressin and two associated proteins, neurophysin 2 and the glycopeptide copeptin. Vasopressin, together with its carrier protein neurophysin II, is packaged into neurosecretory vesicles and transported axonally to the nerve endings in the neurohypophysis, where it is either stored or secreted into the bloodstream. Vasopressin acts as a growth factor by enhancing pH regulation through acid-base transport systems. It has a direct antidiuretic action on the kidney and also causes vasoconstriction of the peripheral vessels. Vasopressin can also contract smooth muscle during parturition and lactation. It also plays a role in cognition, tolerance, adaptation and complex sexual and maternal behaviour, as well as in the regulation of water excretion and cardiovascular functions. Mutations in the vasopressin precursor cause autosomal dominant neurohypophyseal diabetes insipidus (ADNDI), which is characterised by persistant thirst, polydipsia and polyuria.
    • Cellular localizationSecreted
    • Database links
    • Alternative names
      • ADH antibody
      • Antidiuretic hormone antibody
      • Arginine vasopressin neurophysin II antibody
      • ARVP antibody
      • AVP antibody
      • AVP NPII antibody
      • copeptin antibody
      • Vasopressin neurophysin II copeptin antibody
      • VP antibody
      see all

    Anti-Vasopressin antibody images

    • ab68669 staining Vasopressin in sheep brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, permeablized with 0.3% Triton X-100, blocked with 5% serum for 1 hour at 23°C and antigen retrieval was by heat mediation in 0.1M citrate buffer pH 6.0. The sample was incubated with primary antibody (1/100 in 0.3% Triton X-100) at 4°C for 12 hours. A Biotin-conjugated anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody.

      See Abreview

    • IHC image of Vasopressin staining in human Hippocampus FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab68669 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
    • ELISA using ab68669 at varying antibody concentrations. The black line indicates binding to the vasopressin peptide ab68668.

    References for Anti-Vasopressin antibody (ab68669)

    ab68669 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample Mouse Tissue sections (Brain)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 0.1M Citrate Buffer pH 6.0
    Permeabilization Yes - 0.3% Triton X-100
    Specification Brain
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Fixative Paraformaldehyde
    Username

    Miss. Juliana Garcia da Silva Fonseca

    Verified customer

    Submitted Aug 13 2014

    Application Immunohistochemistry (PFA perfusion fixed frozen sections)
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 0.1M Citrate Buffer pH 6.0
    Sample Sheep Tissue sections (Brain)
    Specification Brain
    Permeabilization Yes - 0.3% Triton X-100
    Fixative Paraformaldehyde
    Username

    Miss. Juliana Garcia da Silva Fonseca

    Verified customer

    Submitted Aug 13 2014

    Thank you for your enquiry.

    Although we produce many in-house antibodies, we obtain other antibodies from a wide range of sources. Therefore, comparison experiments will not often have been done.

    In this instance, none of our Vaso...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"