Overview

  • Product nameAnti-VASP antibody
    See all VASP primary antibodies
  • Description
    Mouse polyclonal to VASP
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human VASP protein (AAH38224.1).

  • Positive control
    • Human spleen tissue lysate. Cell lysate from transfected 293T cells. HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab89829 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 42 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionEna/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation.
  • Tissue specificityHighly expressed in platelets.
  • Sequence similaritiesBelongs to the Ena/VASP family.
    Contains 1 WH1 domain.
  • DomainThe EVH2 domain is comprised of 3 regions. Block A is a thymosin-like domain required for G-actin binding. The KLKR motif within this block is essential for the G-actin binding and for actin polymerization. Block B is required for F-actin binding and subcellular location, and Block C for tetramerization.
    The WH1 domain mediates interaction with XIRP1.
  • Post-translational
    modifications
    Major substrate for cAMP-dependent (PKA) and cGMP-dependent protein kinase (PKG) in platelets. The preferred site for PKA is Ser-157, the preferred site for PKG, Ser-239. In ADP-activated platelets, phosphorylation by PKA or PKG on Ser-157 leads to fibrinogen receptor inhibition. Phosphorylation on Thr-278 requires prior phosphorylation on Ser-157 and Ser-239. In response to phorbol ester (PMA) stimulation, phosphorylated by PKC/PRKCA. In response to thrombin, phosphorylated by both PKC and ROCK1. Phosphorylation at Thr-278 by AMPK does not require prior phosphorylation at Ser-157 or Ser-239. Phosphorylation modulates F-actin binding, actin filament elongation and platelet activation. Carbon monoxide (CO) promotes phosphorylation at Ser-157, while nitric oxide (NO) promotes phosphorylation at Ser-157, but also at Ser-239. Response to NO and CO is blunted in platelets from diabetic patients, and VASP is not phosphorylated efficiently at Ser-157 and Ser-239.
  • Cellular localizationCytoplasm. Cytoplasm > cytoskeleton. Cell junction > focal adhesion. Cell projection > lamellipodium membrane. Cell projection > filopodium membrane. Targeted to stress fibers and focal adhesions through interaction with a number of proteins including MRL family members. Localizes to the plasma membrane in protruding lamellipodia and filopodial tips. Stimulation by thrombin or PMA, also translocates VASP to focal adhesions. Localized along the sides of actin filaments throughout the peripheral cytoplasm under basal conditions.
  • Information by UniProt
  • Database links
  • Alternative names
    • Vasodilator stimulated phosphoprotein antibody
    • Vasodilator-stimulated phosphoprotein antibody
    • VASP antibody
    • VASP_HUMAN antibody

Anti-VASP antibody images

  • Anti-VASP antibody (ab89829) at 1 µg/ml + human spleen tissue lysate at 50 µg
    Developed using the ECL technique

    Predicted band size : 42 kDa
    Observed band size : 42 kDa
  • All lanes : Anti-VASP antibody (ab89829) at 1 µg/ml

    Lane 1 : cell lysate from VASP transfected 293T cells
    Lane 2 : cell lysate from non transfected 293T cells

    Lysates/proteins at 25 µg per lane.

    Developed using the ECL technique

    Predicted band size : 42 kDa
    Observed band size : 42 kDa
  • ab89829 at 10 µg/ml staining VASP in paraformaldehyde fixed, permeabilised HeLa cells by immunofluorescence.

References for Anti-VASP antibody (ab89829)

ab89829 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab89829.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"