A synthetic phosphopeptide corresponding to amino acids surrounding the Ser157 phosphorylation site of human VASP
Our Abpromise guarantee covers the use of ab58555 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 0.5µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|WB||Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 42 kDa.|
|IP||Use 1-10µg for 106 cells.|
|ELISA||Use a concentration of 0.05 µg/ml.|
|ICC/IF||Use a concentration of 1 - 10 µg/ml. For immunocytochemistry, fixation protocol may include up to 0.5% formaldehyde.|