Anti-Vav proteins (phospho Y174) antibody (ab47282)


  • Product name
    Anti-Vav proteins (phospho Y174) antibody
    See all Vav proteins primary antibodies
  • Description
    Rabbit polyclonal to Vav proteins (phospho Y174)
  • Specificity
    This antibody is specific for Vav protein, only when phosphorylated at tyrosine 174.
  • Tested applications
    Suitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthesized phosphopeptide derived from human Vav proteins around the phosphorylation site of tyrosine 174 (E-I-YP-E-D)

  • Positive control
    • Jurkat cell extracts


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab47282 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution. See Abreview.
WB 1/500 - 1/1000. Detects a band of approximately 118 kDa.
ELISA 1/10000.


  • Relevance
    The Vav family are Rho/Rac guanosine nucleotide exchange factors (GEFs), consisting of three members in mammalian cells (Vav, Vav2, Vav3) and one in nematodes (CelVav). First discovered based on its transforming properties, Vav is expressed mainly in hematopoietic cells and a few non-hematopoietic tissues, such as the pancreas and tooth enamels. As a signalling transducer, Vav is involved in T-cell activated transduction of T-cell antigen receptor (TCR). T-cell stimulated and tyrosine phosphorylated Vav acts as a catalyst in the exchange of guanosine nucleotides on Rac-1, a GTP binding protein. Using a mouse model, Vav expression has been determined to play an essential role in the cyctosketetal, proliferative, and apoptotic pathways for developing lymphoid cells and its signal response.
  • Cellular localization
    Cytoplasmic and Plasma membrane VAV1, Cytoplasm - VAV2 & VAV3
  • Database links
  • Alternative names
    • 2810040F13Rik antibody
    • A530094I06Rik antibody
    • AA986410 antibody
    • AI847175 antibody
    • FLJ40431 antibody
    • GDP/GTP exchange factor VAV2 antibody
    • Guanine nucleotide exchange factor VAV2 antibody
    • Guanine nucleotide exchange factor VAV3 antibody
    • MGC27838 antibody
    • Oncogene VAV antibody
    • Oncogene VAV1 antibody
    • Oncogene VAV2 antibody
    • Oncogene VAV3 antibody
    • p95 antibody
    • p95vav antibody
    • Protein vav 1 antibody
    • Protein vav 2 antibody
    • Protein vav 3 antibody
    • Proto oncogene vav antibody
    • RGD1565941 antibody
    • VAV antibody
    • vav 1 guanine nucleotide exchange factor antibody
    • Vav 1 oncogene antibody
    • vav 2 guanine nucleotide exchange factor antibody
    • Vav 2 oncogene antibody
    • vav 3 guanine nucleotide exchange factor antibody
    • Vav 3 oncogene antibody
    • vav-T antibody
    • VAV1 oncogene antibody
    • VAV2 oncogene antibody
    • VAV3 oncogene antibody
    see all


  • All lanes : Anti-Vav proteins (phospho Y174) antibody (ab47282) at 1/500 dilution

    Lane 1 : Extrats from Jurkat cells treated with UV
    Lane 2 : Extrats from Jurkat cells untreated

    Lysates/proteins at 30 µg per lane.


This product has been referenced in:
  • Kumar V  et al. Integrin beta8 (ITGB8) activates VAV-RAC1 signaling via FAK in the acquisition of endometrial epithelial cell receptivity for blastocyst implantation. Sci Rep 7:1885 (2017). Read more (PubMed: 28507287) »
  • Kwon HJ  et al. Stepwise phosphorylation of p65 promotes NF-?B activation and NK cell responses during target cell recognition. Nat Commun 7:11686 (2016). Read more (PubMed: 27221592) »

See all 9 Publications for this product

Customer reviews and Q&As

I would not worry about results where the observed molecular weights are 10 or even 15% above the expected molecular weights. As explained in our, this is very common and explained by the post-transl...

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Thank you for your phone call. As I mentioned, because these are phospho-specific antibodies, we recommend using BSA as a blocking agent instead of milk. If this does not help to improve your results, please let me knowyour original order or PO numbera...

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The difference between these two products is that ab76225 is a rabbit monoclonal antibody while ab47282 is a rabbit polyclonal antibody. Both of these antibodies were raised against the corresponding residues around the phophorylated site at the 174 am...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Human Cell lysate - whole cell (platelet)
Loading amount
15 µg
MnCl2/PP2 15 min
Gel Running Conditions
Reduced Denaturing (7.5 % gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C

Abcam user community

Verified customer

Submitted May 29 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Immunocytochemistry/ Immunofluorescence
Human Cell (Platelet)
Yes - 0.2% Triton X-100
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C

Abcam user community

Verified customer

Submitted May 26 2009


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