Anti-Vav proteins (phospho Y174) antibody (ab47282)
Key features and details
- Rabbit polyclonal to Vav proteins (phospho Y174)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Vav proteins (phospho Y174) antibody
See all Vav proteins primary antibodies -
Description
Rabbit polyclonal to Vav proteins (phospho Y174) -
Host species
Rabbit -
Specificity
This antibody is specific for Vav protein, only when phosphorylated at tyrosine 174. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Vav proteins aa 100-200 (phospho Y174).
Database link: P15498 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab47282 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/500 - 1/1000. Detects a band of approximately 118 kDa.
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 118 kDa. |
Target
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Relevance
The Vav family are Rho/Rac guanosine nucleotide exchange factors (GEFs), consisting of three members in mammalian cells (Vav, Vav2, Vav3) and one in nematodes (CelVav). First discovered based on its transforming properties, Vav is expressed mainly in hematopoietic cells and a few non-hematopoietic tissues, such as the pancreas and tooth enamels. As a signalling transducer, Vav is involved in T-cell activated transduction of T-cell antigen receptor (TCR). T-cell stimulated and tyrosine phosphorylated Vav acts as a catalyst in the exchange of guanosine nucleotides on Rac-1, a GTP binding protein. Using a mouse model, Vav expression has been determined to play an essential role in the cyctosketetal, proliferative, and apoptotic pathways for developing lymphoid cells and its signal response. -
Cellular localization
Cytoplasmic and Plasma membrane VAV1, Cytoplasm - VAV2 & VAV3 -
Database links
- Entrez Gene: 10451 Human
- Entrez Gene: 7409 Human
- Entrez Gene: 7410 Human
- Omim: 164875 Human
- Omim: 600428 Human
- Omim: 605541 Human
- SwissProt: P15498 Human
- SwissProt: P52735 Human
see all -
Alternative names
- 2810040F13Rik antibody
- A530094I06Rik antibody
- AA986410 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (12)
ab47282 has been referenced in 12 publications.
- Rossatti P et al. Rapid increase in transferrin receptor recycling promotes adhesion during T cell activation. BMC Biol 20:189 (2022). PubMed: 36002835
- Guo M et al. Inhibition of allogeneic islet graft rejection by VISTA-conjugated liposome. Biochem Biophys Res Commun 516:914-920 (2019). PubMed: 31272717
- Schwartz SL et al. Differential mast cell outcomes are sensitive to FceRI-Syk binding kinetics. Mol Biol Cell 28:3397-3414 (2017). PubMed: 28855374
- Kumar V et al. Integrin beta8 (ITGB8) activates VAV-RAC1 signaling via FAK in the acquisition of endometrial epithelial cell receptivity for blastocyst implantation. Sci Rep 7:1885 (2017). PubMed: 28507287
- Kwon HJ et al. Stepwise phosphorylation of p65 promotes NF-?B activation and NK cell responses during target cell recognition. Nat Commun 7:11686 (2016). PubMed: 27221592