The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Peptide ELISA: antibody detection limit dilution >1/32000.
WB: Use at a concentration of 1 - 1.5 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Guanine nucleotide exchange factor for the Rho family of Ras-related GTPases. Plays an important role in angiogenesis. Its recruitement by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly.