Overview

  • Product nameAnti-VAV2 antibody
    See all VAV2 primary antibodies
  • Description
    Rabbit polyclonal to VAV2
  • Specificityab86699 detects VAV2 in human A431 cells and detects several VAV isoforms in Jurkat cells. The immunogen sequence has significant homology to the conserved site in VAV1 and VAV3.
  • Tested applicationsSuitable for: WB, IP, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rabbit, Human
    Predicted to work with: Rat, Chicken, Orangutan
  • Immunogen

    Synthetic peptide:

    EDEGDDIYED

    conjugated to KLH, corresponding to amino acids 165-174 of Mouse VAV2.

  • Positive control
    • NP40 permeabilized rabbit spleen fibroblasts and A431 cells treated with EGF.

Properties

Applications

Our Abpromise guarantee covers the use of ab86699 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).
IP 1/100.
ICC/IF 1/50.
ELISA 1/2000.

Target

  • FunctionGuanine nucleotide exchange factor for the Rho family of Ras-related GTPases. Plays an important role in angiogenesis. Its recruitement by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly.
  • Tissue specificityWidely expressed.
  • Sequence similaritiesContains 1 CH (calponin-homology) domain.
    Contains 1 DH (DBL-homology) domain.
    Contains 1 PH domain.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 SH2 domain.
    Contains 2 SH3 domains.
  • Information by UniProt
  • Database links
  • Alternative names
    • Guanine nucleotide exchange factor VAV2 antibody
    • Oncogene VAV2 antibody
    • Protein vav 2 antibody
    • VAV 2 antibody
    • Vav 2 oncogene antibody
    • VAV-2 antibody
    • VAV2 antibody
    • VAV2 oncogene antibody
    • VAV2_HUMAN antibody
    see all

Anti-VAV2 antibody images

  • All lanes : Anti-VAV2 antibody (ab86699) at 1/1000 dilution

    Lane 1 : A431 cells treated with EGF
    Lane 2 : A431 cells treated with EGF, exposed to alkaline phosphatase


    Predicted band size : 100 kDa
    Observed band size : 100 kDa
    Additional bands at : <100 kDa. We are unsure as to the identity of these extra bands.
  • Immunocytochemical labeling of VAV2 in paraformaldehyde-fixed and NP40 permeabilized rabbit spleen fibroblasts. The cells were labeled with ab86699 at a 1/50 dilution and detected using an appropriate secondary antibody conjugated to Cy3.

References for Anti-VAV2 antibody (ab86699)

ab86699 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"