Anti-VAV2 (phospho Y142) antibody (ab73707)

Overview

  • Product nameAnti-VAV2 (phospho Y142) antibody
    See all VAV2 primary antibodies
  • Description
    Rabbit polyclonal to VAV2 (phospho Y142)
  • SpecificityThis antibody detects endogenous levels of VAV2 only when phosphorylated at tyrosine 142.
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    A synthesized phosphopeptide derived from human VAV2 around the phosphorylation site of tyrosine 142 (D-V-YP-R-S).

  • Positive control
    • Human brain tissue sections, NIH/3T3 cells and extracts from K562 cells and Hela cells, treated with TNF.

Properties

Applications

Our Abpromise guarantee covers the use of ab73707 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 101 kDa (predicted molecular weight: 101 kDa).
ELISA 1/5000.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/500 - 1/1000.

Target

  • FunctionGuanine nucleotide exchange factor for the Rho family of Ras-related GTPases. Plays an important role in angiogenesis. Its recruitement by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly.
  • Tissue specificityWidely expressed.
  • Sequence similaritiesContains 1 CH (calponin-homology) domain.
    Contains 1 DH (DBL-homology) domain.
    Contains 1 PH domain.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 SH2 domain.
    Contains 2 SH3 domains.
  • Information by UniProt
  • Database links
  • Alternative names
    • Guanine nucleotide exchange factor VAV2 antibody
    • Oncogene VAV2 antibody
    • Protein vav 2 antibody
    • VAV 2 antibody
    • Vav 2 oncogene antibody
    • VAV-2 antibody
    • VAV2 antibody
    • VAV2 oncogene antibody
    • VAV2_HUMAN antibody
    see all

Anti-VAV2 (phospho Y142) antibody images

  • All lanes : Anti-VAV2 (phospho Y142) antibody (ab73707) at 1/500 dilution

    Lane 1 : Extracts from K562 cells treated with TNF (200ng/ml, 30mins)
    Lane 2 : Extracts from Hela cells treated with TNF (200ng/ml, 30mins)
    Lane 3 : Extracts from K562 cells treated with TNF (200ng/ml, 30mins) plus 5µg immunizing phosphopeptide

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 101 kDa
    Observed band size : 101 kDa
  • Immunohistochemistry analysis of paraffin-embedded human brain tissue using ab73707 at a 1:50 dilution.

    Left image un-treated.

    Right image treated with immunizing phosphopeptide.

  • Immunofluorescence analysis of NIH/3T3 cells using ab73707 at a 1:500 dilution.

    Left image un-treated.

    Right image treated with immunizing phosphopeptide.

References for Anti-VAV2 (phospho Y142) antibody (ab73707)

ab73707 has not yet been referenced specifically in any publications.

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