Overview

  • Product nameAnti-VAV2 (phospho Y172) antibody
    See all VAV2 primary antibodies
  • Description
    Rabbit polyclonal to VAV2 (phospho Y172)
  • Specificityab86695 detects Vav1, Vav2, and Vav3 in Jurkat cells treated with pervanadate. The antibody does not detect these Vavs after treatment with alkaline phosphatase.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide conjugated to KLH corresponding to amino acid residues surrounding Tyr-172 in human VAV2. This sequence has high homology with similar regions in rat and mouse VAV2, and has significant homology to the conserved site in Vav1 and Vav3.

  • Positive control
    • A431 cells treated with EGF.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab86695 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 101 kDa (predicted molecular weight: 101 kDa).
ELISA 1/2000.

Target

  • FunctionGuanine nucleotide exchange factor for the Rho family of Ras-related GTPases. Plays an important role in angiogenesis. Its recruitement by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly.
  • Tissue specificityWidely expressed.
  • Sequence similaritiesContains 1 CH (calponin-homology) domain.
    Contains 1 DH (DBL-homology) domain.
    Contains 1 PH domain.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 SH2 domain.
    Contains 2 SH3 domains.
  • Information by UniProt
  • Database links
  • Alternative names
    • Guanine nucleotide exchange factor VAV2 antibody
    • Oncogene VAV2 antibody
    • Protein vav 2 antibody
    • VAV 2 antibody
    • Vav 2 oncogene antibody
    • VAV-2 antibody
    • VAV2 antibody
    • VAV2 oncogene antibody
    • VAV2_HUMAN antibody
    see all

Anti-VAV2 (phospho Y172) antibody images

  • All lanes : Anti-VAV2 (phospho Y172) antibody (ab86695) at 1/1000 dilution

    Lane 1 : A431 cells treated with EGF
    Lane 2 : A431 cells treated with EGF, exposed to alkaline phosphatase


    Predicted band size : 101 kDa
    Observed band size : 101 kDa
    Additional bands at : <100 kDa. We are unsure as to the identity of these extra bands.

References for Anti-VAV2 (phospho Y172) antibody (ab86695)

This product has been referenced in:
  • Espejo R  et al. PTP-PEST targets a novel tyrosine site in p120 catenin to control epithelial cell motility and Rho GTPase activity. J Cell Sci 127:497-508 (2014). Read more (PubMed: 24284071) »
  • Havel LS  et al. Vimentin regulates lung cancer cell adhesion through a VAV2-Rac1 pathway to control focal adhesion kinase activity. Oncogene N/A:N/A (2014). Human . Read more (PubMed: 24858039) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"