The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 98 kDa.
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionExchange factor for GTP-binding proteins RhoA, RhoG and, to a lesser extent, Rac1. Binds physically to the nucleotide-free states of those GTPases. Plays an important role in angiogenesis. Its recruitement by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly.
Immunohistochemistry analysis (formalin-fixed, paraffin-embedded)of Human Skin stained with ab118904 at 5 ug/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Immunohistochemistry analysis (formalin-fixed, paraffin-embedded)of Human Spleen stained with ab118904 at 5 ug/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Western blot - Anti-VAV3 antibody (ab118904)
Anti-VAV3 antibody (ab118904) at 0.3 µg/ml + Human bone marrow lysate at 35 µg developed using the ECL technique
Predicted band size : 98 kDa
Exposure time : 1 hour
References for Anti-VAV3 antibody (ab118904)
has not yet been referenced specifically in any publications.
Publishing research using ab118904? Please let us know so that we can cite the reference in this datasheet.
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