Anti-VAV3 antibody [EP1130Y] (ab52938)


  • Product name
    Anti-VAV3 antibody [EP1130Y]
    See all VAV3 primary antibodies
  • Description
    Rabbit monoclonal [EP1130Y] to VAV3
  • Tested applications
    Suitable for: WB, IP, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A synthetic peptide corresponding to residues near the C-term of human Vav3.

  • Positive control
    • Jurkat cell lysate; permeabalized Jurkat cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.


    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


    Alternative versions available:

    Anti-VAV3 antibody (Alexa Fluor® 647) [EP1130Y] (ab206236)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents



Our Abpromise guarantee covers the use of ab52938 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 98 kDa (predicted molecular weight: 98 kDa).
IP 1/50.
Flow Cyt 1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration. PubMed: 23566222

Methanol fixed cells

  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      Exchange factor for GTP-binding proteins RhoA, RhoG and, to a lesser extent, Rac1. Binds physically to the nucleotide-free states of those GTPases. Plays an important role in angiogenesis. Its recruitement by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly.
    • Sequence similarities
      Contains 1 CH (calponin-homology) domain.
      Contains 1 DH (DBL-homology) domain.
      Contains 1 PH domain.
      Contains 1 phorbol-ester/DAG-type zinc finger.
      Contains 1 SH2 domain.
      Contains 2 SH3 domains.
    • Information by UniProt
    • Database links
    • Alternative names
      • FLJ40431 antibody
      • Guanine nucleotide exchange factor VAV3 antibody
      • Protein vav 3 antibody
      • Protein vav3 antibody
      • RGD1565941 antibody
      • VAV 3 antibody
      • Vav 3 guanine nucleotide exchange factor antibody
      • VAV 3 oncogene antibody
      • VAV 3 protein antibody
      • VAV-3 antibody
      • Vav3 antibody
      • VAV3 oncogene antibody
      • VAV3 protein antibody
      • VAV3_HUMAN antibody
      see all

    Anti-VAV3 antibody [EP1130Y] images

    • Anti-VAV3 antibody [EP1130Y] (ab52938) at 1/100000 dilution + Jurkat cell lysate at 10 µg

      HRP-labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 98 kDa
      Observed band size : ~98 kDa (why is the actual band size different from the predicted?)
    • Flow cytometric analysis of permeabilized Jurkat cells using ab52938 (red) or a rabbit IgG (negative) (green).

    References for Anti-VAV3 antibody [EP1130Y] (ab52938)

    This product has been referenced in:

    See all 4 Publications for this product

    Product Wall

    Thanks for the reply. I am a little stumped as to why you are having these issues. I can only think of 2 things that may help (and I am sure you have already tried them): 1 - One reason for multiply bands is that there is not enough protease inhibitor ...

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