Recombinant Anti-VAV3 (phospho Y173) antibody [EPR1068A] (ab109544)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1068A] to VAV3 (phospho Y173)
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-VAV3 (phospho Y173) antibody [EPR1068A]
See all VAV3 primary antibodies -
Description
Rabbit monoclonal [EPR1068A] to VAV3 (phospho Y173) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- Jurkat cell lysates
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1068A -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109544 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 98 kDa.
|
Notes |
---|
WB
1/1000 - 1/10000. Predicted molecular weight: 98 kDa. |
Target
-
Function
Exchange factor for GTP-binding proteins RhoA, RhoG and, to a lesser extent, Rac1. Binds physically to the nucleotide-free states of those GTPases. Plays an important role in angiogenesis. Its recruitement by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly. -
Sequence similarities
Contains 1 CH (calponin-homology) domain.
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 SH2 domain.
Contains 2 SH3 domains. - Information by UniProt
-
Database links
- Entrez Gene: 10451 Human
- Entrez Gene: 57257 Mouse
- Omim: 605541 Human
- SwissProt: Q9UKW4 Human
- SwissProt: Q9R0C8 Mouse
- Unigene: 267659 Human
- Unigene: 282257 Mouse
-
Alternative names
- FLJ40431 antibody
- Guanine nucleotide exchange factor VAV3 antibody
- Protein vav 3 antibody
see all
Images
Datasheets and documents
-
SDS download
-
Datasheet download
References (3)
ab109544 has been referenced in 3 publications.
- Nayak RC et al. Nuclear Vav3 is required for polycomb repression complex-1 activity in B-cell lymphoblastic leukemogenesis. Nat Commun 13:3056 (2022). PubMed: 35650206
- Maruyama K et al. Bone-protective Functions of Netrin 1 Protein. J Biol Chem 291:23854-23868 (2016). PubMed: 27681594
- Kovárová D et al. Downregulation of HOPX controls metastatic behavior in sarcoma cells and identifies genes associated with metastasis. Mol Cancer Res 11:1235-47 (2013). PubMed: 23938949