Overview

  • Product nameAnti-VAX1 antibody
    See all VAX1 primary antibodies
  • Description
    Mouse monoclonal to VAX1
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment: MFGKPDKMDV RCHSDAEAAR VSKNAHKESR ESKGAEGNLP AAFLKEPQGA FSASGAAEDC NKSKSNSAAD PDYCRRILVR DAKGSIREII LPKGLDLDRP, corresponding to amino acids 1-100 of Human VAX1 (NP_954582) with a proprietary tag.

  • Positive control
    • Tagged recombinant Human VAX1 fragment (the immunogen). PC-12, HepG2, Raw 264.7 and NIH/3T3 cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab88424 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 35 kDa.
ELISA Use at an assay dependent dilution.

Target

Anti-VAX1 antibody images

  • Anti-VAX1 antibody (ab88424) at 1 µg/ml + Tagged recombinant Human VAX1 fragment (the immunogen) at 0.1 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 35 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • Anti-VAX1 antibody (ab88424) at 1 µg/ml + PC-12 cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 35 kDa
    Observed band size : 35 kDa
  • Anti-VAX1 antibody (ab88424) at 1 µg/ml + HepG2 cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 35 kDa
    Observed band size : 35 kDa
  • Anti-VAX1 antibody (ab88424) at 1 µg/ml + Raw 264.7 cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 35 kDa
    Observed band size : 35 kDa
  • Anti-VAX1 antibody (ab88424) at 1 µg/ml + NIH/3T3 cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 35 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)

References for Anti-VAX1 antibody (ab88424)

ab88424 has not yet been referenced specifically in any publications.

Product Wall

Thanks for the reply.

I have processed you request to get ab87257 as a replacement antibody. It is out of stock at the moment, but we expect to get it back in stock around 3rd April and we will get it to you as soon as possible. Your new orde...

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Thank you for your reply.

The order information you provided does not have the details for the Vax1 antibody, ab88424. I did find an order for this antibody from **********, it was to the attention of *******. Would that be the correct order?...

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Thank you for contacting Abcam about ab88424.

The antibody has been tested in Western blot against VAX1 transfected cell lysate and also against the recombinant protein, both giving good results. Therefore we are confident that the antibody d...

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Thank you for contacting us and for bringing this to our attention.

Would you be able to provide me with the details of your test as well as data. This information will be extremely valuable for us for for internal testing of this product. ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"