Recombinant Anti-VAX2 antibody [EPR10424] (ab154855)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10424] to VAX2
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-VAX2 antibody [EPR10424]
See all VAX2 primary antibodies -
Description
Rabbit monoclonal [EPR10424] to VAX2 -
Host species
Rabbit -
Specificity
The alignment of the immunogen sequence of ab154855 and human and rat VAX1 shows a high homology of 92% (human) and 93% (rat). Based on the sequence, a cross-reaction of ab154855 with VAX1 is very likely, although this has not been experimentally tested by us. -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Jurkat, HeLa, Y79 cell lysates; Y79 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10424 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154855 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 31 kDa.
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ICC/IF |
1/100 - 1/250.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 31 kDa. |
ICC/IF
1/100 - 1/250. |
Target
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Relevance
VAX2 belongs to the EMX homeobox family and contains 1 homeobox DNA-binding domain. VAX2 gene has high sequence similarity to VAX1 gene and is almost exclusively expressed in the ventral portion of the retina during development. It may play an important role in morphogenetic processes underlying the correct development of the eye, particularly in the establishment of a correct dorsoventral pattern and may be a regulator of axial polarization in the retina. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 25806 Human
- Entrez Gene: 64572 Rat
- GenBank: NP_036608.1 Human
- Omim: 604295 Human
- SwissProt: Q9UIW0 Human
- SwissProt: Q9JLZ9 Rat
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Alternative names
- DRES93 antibody
- VAX2 antibody
- Ventral anterior homeobox 2 antibody
Images
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All lanes : Anti-VAX2 antibody [EPR10424] (ab154855) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Y79 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 31 kDa -
Immunofluorescent analysis of Y79 cells labeling VAX2 with ab154855 at 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab154855 has not yet been referenced specifically in any publications.