Recombinant
RabMAb

Anti-VAX2 antibody [EPR10424] (ab154855)

Overview

  • Product name
    Anti-VAX2 antibody [EPR10424]
    See all VAX2 primary antibodies
  • Description
    Rabbit monoclonal [EPR10424] to VAX2
  • Host species
    Rabbit
  • Specificity
    The alignment of the immunogen sequence of ab154855 and human and rat VAX1 shows a high homology of 92% (human) and 93% (rat). Based on the sequence, a cross-reaction of ab154855 with VAX1 is very likely, although this has not been experimentally tested by us.
  • Tested applications
    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human VAX2 (Uniprot ID Q9UIW0).

  • Positive control
    • Jurkat, HeLa, Y79 cell lysates; Y79 cells.
  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab154855 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 31 kDa.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Relevance
      VAX2 belongs to the EMX homeobox family and contains 1 homeobox DNA-binding domain. VAX2 gene has high sequence similarity to VAX1 gene and is almost exclusively expressed in the ventral portion of the retina during development. It may play an important role in morphogenetic processes underlying the correct development of the eye, particularly in the establishment of a correct dorsoventral pattern and may be a regulator of axial polarization in the retina.
    • Cellular localization
      Nuclear
    • Database links
    • Alternative names
      • DRES93 antibody
      • VAX2 antibody
      • Ventral anterior homeobox 2 antibody

    Images

    • All lanes : Anti-VAX2 antibody [EPR10424] (ab154855) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Y79 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 31 kDa

    • Immunofluorescent analysis of Y79 cells labeling VAX2 with ab154855 at 1/100 dilution.

    References

    ab154855 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Sample
    Zebrafish Tissue sections (retinal ganglion cells)
    Specification
    retinal ganglion cells
    Permeabilization
    Yes - 0.1% Triton X
    Fixative
    Paraformaldehyde
    Username

    Dr. Ryan Macdonald

    Verified customer

    Submitted Mar 12 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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