Anti-VAX2 antibody [VAX2A8F12] (ab50899)

Overview

  • Product nameAnti-VAX2 antibody [VAX2A8F12]
    See all VAX2 primary antibodies
  • Description
    Mouse monoclonal [VAX2A8F12] to VAX2
  • Tested applicationsSuitable for: ICC/IF, WB, Dot blotmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment (Human)

  • Positive control
    • NIH3T3 whole cell lysate, HeLa whole cell lysate, HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab50899 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/10.
WB 1/50. Predicted molecular weight: 31 kDa.
Dot blot Use at an assay dependent dilution.

Target

  • RelevanceVAX2 belongs to the EMX homeobox family and contains 1 homeobox DNA-binding domain. VAX2 gene has high sequence similarity to VAX1 gene and is almost exclusively expressed in the ventral portion of the retina during development. It may play an important role in morphogenetic processes underlying the correct development of the eye, particularly in the establishment of a correct dorsoventral pattern and may be a regulator of axial polarization in the retina.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • DRES93 antibody
    • VAX2 antibody
    • Ventral anterior homeobox 2 antibody

Anti-VAX2 antibody [VAX2A8F12] images

  • Anti-VAX2 antibody [VAX2A8F12] (ab50899) at 1/50 dilution + HeLa whole cell lysate at 50 µg

    Secondary
    Anti mouse IgG antibody at 1/2500 dilution

    Predicted band size : 31 kDa
    Observed band size : 31 kDa
  • Anti-VAX2 antibody [VAX2A8F12] (ab50899) at 1/50 dilution + NIH3T3 whole cell lysate at 50 µg/ml

    Secondary
    Anti mouse IgG antibody at 1/2500 dilution

    Predicted band size : 31 kDa
    Observed band size : 31 kDa
  • Ab50899 at 1/10 dilution staining HeLa cells; visualised with AlexaFluor®488 Goat Anti-mouse IgG at 1/200 dilution.

References for Anti-VAX2 antibody [VAX2A8F12] (ab50899)

ab50899 has not yet been referenced specifically in any publications.

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We have checked the level of homology between the provided sequence and immunogen sequence of ab50899. Based on this information we do not believe that ab50899 would work for Zebrafish sample because there is almost ...

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