Anti-VCAM1 antibody [1.4C3], prediluted (ab74514)

Overview

  • Product nameAnti-VCAM1 antibody [1.4C3], predilutedSee all VCAM1 primary antibodies ...
  • Description
    Mouse monoclonal [1.4C3] to VCAM1, prediluted
  • Tested applicationsIHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Stimulated human umbilical vein endothelial cells (HUVEC)

  • Positive control
    • Human Placental and tonsil tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab74514 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notesIHC-P: Ready-to-Use. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8.0, for 10-20 min followed by cooling at RT for 20 min.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionImportant in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
    • Tissue specificityExpressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue.
    • Sequence similaritiesContains 7 Ig-like C2-type (immunoglobulin-like) domains.
    • DomainEither the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion.
    • Post-translational
      modifications
      Sialoglycoprotein.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD 106 antibody
      • CD106 antibody
      • CD106 Antigen antibody
      • DKFZp779G2333 antibody
      • INCAM 100 antibody
      • INCAM-100 antibody
      • L1CAM antibody
      • MGC99561 antibody
      • V-CAM 1 antibody
      • Vascular Cell Adhesion Molecule 1 antibody
      • Vascular cell adhesion protein 1 antibody
      • VCAM 1 antibody
      • VCAM-1 antibody
      • VCAM1 antibody
      • VCAM1_HUMAN antibody
      see all

    Anti-VCAM1 antibody [1.4C3], prediluted images

    • Formalin-fixed, paraffin-embedded human tonsil stained with ab74514 using peroxidase-conjugate and AEC chromogen. Note membrane staining of hematopoietic cells in germinal center.

    References for Anti-VCAM1 antibody [1.4C3], prediluted (ab74514)

    This product has been referenced in:
    • Burrows FJ  et al. Influence of tumor-derived interleukin 1 on melanoma-endothelial cell interactions in vitro. Cancer Res 51:4768-75 (1991). Read more (PubMed: 1680022) »
    • Wellicome SM  et al. A monoclonal antibody that detects a novel antigen on endothelial cells that is induced by tumor necrosis factor, IL-1, or lipopolysaccharide. J Immunol 144:2558-65 (1990). Read more (PubMed: 2108206) »

    See all 3 Publications for this product

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    This antibody is a diluted version of antibody at concentration 200ug/ml. When diluted to 7ml for ready to use purpose the concentration comes out to be 28.5ug/ml. Please use the isotype control accordingly.
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    I am sorry these products did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued three free of charge replacements. To check the status of the order please contact our Customer Service team...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"