Anti-VCAM1 antibody [1.G11B1] (Phycoerythrin) (ab82438)

Overview

  • Product name
    Anti-VCAM1 antibody [1.G11B1] (Phycoerythrin)
    See all VCAM1 primary antibodies
  • Description
    Mouse monoclonal [1.G11B1] to VCAM1 (Phycoerythrin)
  • Conjugation
    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Specificity
    ab82438 inhibits in vitro binding of lymphocytes and monocytes to VCAM1 on stimulated endothelium.
  • Tested applications
    Suitable for: WB, ELISA, IHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

Applications

Our Abpromise guarantee covers the use of ab82438 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 81 kDa.
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use 10µl for 106 cells. ab91357-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
  • Tissue specificity
    Expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue.
  • Sequence similarities
    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
  • Domain
    Either the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion.
  • Post-translational
    modifications
    Sialoglycoprotein.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD106 antibody
    • CD106 Antigen antibody
    • INCAM 100 antibody
    • INCAM-100 antibody
    • L1CAM antibody
    • MGC99561 antibody
    • V-CAM 1 antibody
    • Vascular Cell Adhesion Molecule 1 antibody
    • Vascular cell adhesion protein 1 antibody
    • VCAM 1 antibody
    • VCAM-1 antibody
    • VCAM1 antibody
    • VCAM1_HUMAN antibody
    see all

Images

  • Flow cytometry analysis staining VCAM1 in HU-VEC-C cells using ab82438 at a dilution of 10 μL/106 cells. Mouse IgG1-PE was used as an isotype control.

References

ab82438 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human pluripotent SC derived epicardial like cells)
Permeabilization
Yes - Saponin
Specification
Human pluripotent SC derived epicardial like cells
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 4% · Temperature: 4°C
Fixative
Paraformaldehyde
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Submitted Oct 31 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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