Overview

  • Product name
    Anti-VCAM1 antibody [M/K-2]
    See all VCAM1 primary antibodies
  • Description
    Rat monoclonal [M/K-2] to VCAM1
  • Specificity
    Present on the +/+ 2.4 stromal cell clone, large cells in bone marrow, and a subset of thymic macrophages. The stromal cell antigen is also detectable on the EOMA and BMS2 endothelial cell lines. Reacts with all known isoforms of murine VCAM-1. Murine vascular adhesion molecule (VCAM1), single chain glycoprotein of Mr100-kDa (under reducing conditions).
  • Tested applications
    Suitable for: IHC-Fr, IP, ICC/IF, Functional Studiesmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    BALB/3T3 and +/+ 2.4 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab19569 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Immunoprecipitation of the VCAM1 integrin complex.

ICC/IF Use at an assay dependent concentration.
Functional Studies Use at an assay dependent concentration.

Studies of cell-cell interaction. Cell adhesion assays. Inhibition of lymphopoiesis in Whitlock-Witte long term marrow cultures.

  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
    • Tissue specificity
      Expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue.
    • Sequence similarities
      Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
    • Domain
      Either the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion.
    • Post-translational
      modifications
      Sialoglycoprotein.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD106 antibody
      • CD106 Antigen antibody
      • INCAM 100 antibody
      • INCAM-100 antibody
      • L1CAM antibody
      • MGC99561 antibody
      • V-CAM 1 antibody
      • Vascular Cell Adhesion Molecule 1 antibody
      • Vascular cell adhesion protein 1 antibody
      • VCAM 1 antibody
      • VCAM-1 antibody
      • VCAM1 antibody
      • VCAM1_HUMAN antibody
      see all

    Images

    • ab19569 at a 1/1000 diution staining VCAM1 in mouse brain tissue by Immunohistochemistry (frozen sections), incubated for 2 hours at 20°C. PFA fixed. Blocked with 10% serum for 1 hour at 20°C. Secondary used at 1/100 ab6733 Rabbit polyclonal to Rat IgG - H&L (Biotin). DAB staining (brown colour). Counterstain is Chresyl violet.

      See Abreview

    References

    This product has been referenced in:
    • Dan Z  et al. A pH-Responsive Host-guest Nanosystem Loading Succinobucol Suppresses Lung Metastasis of Breast Cancer. Theranostics 6:435-45 (2016). Read more (PubMed: 26909117) »
    • Chen PY  et al. Endothelial-to-mesenchymal transition drives atherosclerosis progression. J Clin Invest 125:4514-28 (2015). Read more (PubMed: 26517696) »

    See all 5 Publications for this product

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Mouse brain)
    Specification
    Mouse brain
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Username

    Dr. Thomas Weissensteiner

    Verified customer

    Submitted Feb 18 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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