Anti-VCAM1 antibody [M/K-2] (Biotin) (ab24942)

Overview

  • Product nameAnti-VCAM1 antibody [M/K-2] (Biotin)
    See all VCAM1 primary antibodies
  • Description
    Rat monoclonal [M/K-2] to VCAM1 (Biotin)
  • ConjugationBiotin
  • Tested applicationsSuitable for: Blocking, Inhibition Assay, Flow Cyt, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    The details of the immunogen for this antibody are not available.

Applications

Our Abpromise guarantee covers the use of ab24942 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Blocking Use at an assay dependent concentration.

Blocks lymphocyte adhesion in Whitlock-Witte long-term bone marrow cultures.

Inhibition Assay Use at an assay dependent concentration.

Inhibition of lymphopoiesis in Whitlock-Witte cultures.

Flow Cyt Use 1µg for 106 cells.

ab18403 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IP Use at an assay dependent concentration.

Immunoprecipitates a peptide that gives a single band on SDS PAGE gels with an apparent Mr of ~100 kDa under reducing conditions and 92 kDa under non reducing conditions.

ICC/IF Use at an assay dependent concentration.

Target

  • FunctionImportant in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
  • Tissue specificityExpressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue.
  • Sequence similaritiesContains 7 Ig-like C2-type (immunoglobulin-like) domains.
  • DomainEither the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion.
  • Post-translational
    modifications
    Sialoglycoprotein.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD106 antibody
    • CD106 Antigen antibody
    • INCAM 100 antibody
    • INCAM-100 antibody
    • L1CAM antibody
    • MGC99561 antibody
    • V-CAM 1 antibody
    • Vascular Cell Adhesion Molecule 1 antibody
    • Vascular cell adhesion protein 1 antibody
    • VCAM 1 antibody
    • VCAM-1 antibody
    • VCAM1 antibody
    • VCAM1_HUMAN antibody
    see all

References for Anti-VCAM1 antibody [M/K-2] (Biotin) (ab24942)

ab24942 has not yet been referenced specifically in any publications.

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