Overview

  • Product nameAnti-VCAM1 antibody [M/K-2] (Phycoerythrin)See all VCAM1 primary antibodies ...
  • Description
    Rat monoclonal [M/K-2] to VCAM1 (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • Specificityab25452 recognises CD106/VCAM1 (100 kDa)
  • Tested applicationsFlow Cyt, ICC/IF, Blocking, Inhibition Assay, IP more details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • General notes


    The M/K-2 monoclonal antibody has been used in transplant studies to suppress cardiac rejection and induce long term cardiac graft survival. It immunoprecipitates a peptide that gives a single band on SDS-PAGE gels with an apparent Mr of ~100 kDa under reducing conditions and 92 kDa under nonreducing conditions.

Properties

Applications

Our Abpromise guarantee covers the use of ab25452 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Flow Cyt
ICC/IF
Blocking
Inhibition Assay
IP
  • Application notesBL: Use at an assay dependent dilution.
    ab25452 blocks lymphocyte adhesion in Whitlock Witte long term bone marrow cultures.
    Flow Cyt: Use 0.2µg for 106 cells.
    IF: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.
    ab25452 immunoprecipitates a peptide that gives a single band on SDS PAGE gels with an apparent Mr of 100 kDa under reducing conditions and 92 kDa under nonreducing conditions.
    Inhib: Use at an assay dependent dilution.
    ab25452 inhibits lymphopoiesis in Whitlock Witte cultures.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionImportant in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
    • Tissue specificityExpressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue.
    • Sequence similaritiesContains 7 Ig-like C2-type (immunoglobulin-like) domains.
    • DomainEither the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion.
    • Post-translational
      modifications
      Sialoglycoprotein.
    • Cellular localizationMembrane.
    • Target information above from: UniProt accession P19320 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • CD 106 antibody
      • CD106 antibody
      • CD106 Antigen antibody
      • DKFZp779G2333 antibody
      • INCAM 100 antibody
      • INCAM-100 antibody
      • L1CAM antibody
      • MGC99561 antibody
      • V-CAM 1 antibody
      • Vascular Cell Adhesion Molecule 1 antibody
      • Vascular cell adhesion protein 1 antibody
      • VCAM 1 antibody
      • VCAM-1 antibody
      • VCAM1 antibody
      • VCAM1_HUMAN antibody
      see all

    References for Anti-VCAM1 antibody [M/K-2] (Phycoerythrin) (ab25452)

    This product has been referenced in:
    • Olme CH  et al. Live cell detection of chromosome 2 deletion and Sfpi1/PU1 loss in radiation-induced mouse acute myeloid leukaemia. Leuk Res 37:1374-82 (2013). Flow Cyt ; Mouse . Read more (PubMed: 23806234) »

    See 1 Publication for this product

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