Overview

  • Product name
    Anti-VCAM1 antibody [MVCAM.A(429)]
    See all VCAM1 primary antibodies
  • Description
    Rat monoclonal [MVCAM.A(429)] to VCAM1
  • Tested applications
    Suitable for: Flow Cyt, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue/ cell preparation (Mouse) - Stromal cell line PA6.

  • General notes


    This antibody is reported to partially block VCAM1 mediated functions.

Properties

Applications

Our Abpromise guarantee covers the use of ab27560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/100 - 1/200.

Use 10µl of the suggested working dilution to label 106 cells in 100µl.

ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration.

Target

  • Function
    Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
  • Tissue specificity
    Expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue.
  • Sequence similarities
    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
  • Domain
    Either the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion.
  • Post-translational
    modifications
    Sialoglycoprotein.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD106 antibody
    • CD106 Antigen antibody
    • INCAM 100 antibody
    • INCAM-100 antibody
    • L1CAM antibody
    • MGC99561 antibody
    • V-CAM 1 antibody
    • Vascular Cell Adhesion Molecule 1 antibody
    • Vascular cell adhesion protein 1 antibody
    • VCAM 1 antibody
    • VCAM-1 antibody
    • VCAM1 antibody
    • VCAM1_HUMAN antibody
    see all

References

This product has been referenced in:
  • Horie T  et al. MicroRNA-33 deficiency reduces the progression of atherosclerotic plaque in ApoE-/- mice. J Am Heart Assoc 1:e003376 (2012). Mouse . Read more (PubMed: 23316322) »
  • Mikkelsen HB  et al. The macrophage system in the intestinal muscularis externa during inflammation: an immunohistochemical and quantitative study of osteopetrotic mice. Histochem Cell Biol 130:363-73 (2008). IHC-Fr ; Mouse . Read more (PubMed: 18392842) »

See all 2 Publications for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Username

Abcam user community

Verified customer

Submitted Mar 22 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (ileum (small intestine) treated with lipopolysacch)
Specification
ileum (small intestine) treated with lipopolysacch
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% in TBS
Username

Abcam user community

Verified customer

Submitted May 23 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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