Overview

  • Product nameAnti-VCAM1 antibody [MVCAM.A(429)]See all VCAM1 primary antibodies ...
  • Description
    Rat monoclonal [MVCAM.A(429)] to VCAM1
  • Tested applicationsFlow Cyt, IHC-Fr more details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue/ cell preparation (Mouse) - Stromal cell line PA6.

  • General notes


    This antibody is reported to partially block VCAM1 mediated functions.

Properties

Applications

Our Abpromise guarantee covers the use of ab27560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Flow Cyt 1/100 - 1/200. Use 10µl of the suggested working dilution to label 106 cells in 100µl.
IHC-Fr Use at an assay dependent dilution.

Target

  • FunctionImportant in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
  • Tissue specificityExpressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue.
  • Sequence similaritiesContains 7 Ig-like C2-type (immunoglobulin-like) domains.
  • DomainEither the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion.
  • Post-translational
    modifications
    Sialoglycoprotein.
  • Cellular localizationMembrane.
  • Target information above from: UniProt accession P19320 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • CD 106 antibody
    • CD106 antibody
    • CD106 Antigen antibody
    • DKFZp779G2333 antibody
    • INCAM 100 antibody
    • INCAM-100 antibody
    • L1CAM antibody
    • MGC99561 antibody
    • V-CAM 1 antibody
    • Vascular Cell Adhesion Molecule 1 antibody
    • Vascular cell adhesion protein 1 antibody
    • VCAM 1 antibody
    • VCAM-1 antibody
    • VCAM1 antibody
    • VCAM1_HUMAN antibody
    see all

References for Anti-VCAM1 antibody [MVCAM.A(429)] (ab27560)

This product has been referenced in:
  • Mikkelsen HB  et al. The macrophage system in the intestinal muscularis externa during inflammation: an immunohistochemical and quantitative study of osteopetrotic mice. Histochem Cell Biol 130:363-73 (2008). IHC-Fr ; Mouse . Read more (PubMed: 18392842) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (ileum (small intestine) treated with lipopolysacch)
Specification ileum (small intestine) treated with lipopolysacch
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% in TBS
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Submitted May 23 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"