The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Neat - 1/10 working dilution. Use 10µl of this for a million cells in 100µl.
ab18445-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
FunctionImportant in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
Tissue specificityExpressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue.