VCAM1 (CD106) Human ELISA Kit (ab46118)

Overview

  • Product nameVCAM1 (CD106) Human ELISA Kit
    See all VCAM1 kits
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    A 8 48.79ng/ml 0.221 0.45%
    B 8 5.81ng/ml 0.132 2.27%
    A 8 48.79ng/ml 0.221 0.45%
    B 8 5.81ng/ml 0.132 2.27%
  • Tests
    2 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 0.6 ng/ml
  • Range
    1.56 ng/ml - 50 ng/ml
  • Assay time
    1h 40m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Human VCAM1 (CD106) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of VCAM1 in cell culture supernatants, buffered solutions, serum, plasma, and other body fluids.

    A monoclonal antibody specific for VCAM1 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known VCAM1 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for VCAM1 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of VCAM1 present in the samples.

    This kit will recognize both endogenous and recombinant Human VCAM1.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionImportant in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
  • Tissue specificityExpressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue.
  • Sequence similaritiesContains 7 Ig-like C2-type (immunoglobulin-like) domains.
  • DomainEither the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion.
  • Post-translational
    modifications
    Sialoglycoprotein.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Alternative names
    • CD106
    • CD106 antigen
    • DKFZp779G2333
    • INCAM 100
    • INCAM-100
    • INCAM100
    • L1CAM
    • MGC99561
    • V CAM 1
    • V-CAM 1
    • Vascular cell adhesion molecule 1
    • Vascular cell adhesion protein 1
    • VCAM 1
    • VCAM-1
    • VCAM1
    • VCAM1_HUMAN
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab46118 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

VCAM1 (CD106) Human ELISA Kit images

  • Representative Standard Curve using ab46118.

Protocols

References for VCAM1 (CD106) Human ELISA Kit (ab46118)

ab46118 has not yet been referenced specifically in any publications.

Product Wall

VCAM1 concentration in human plasma

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Human plasma samples were diluted 50 times by diluent buffer.
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Abcam user community

Verified customer

Submitted Jun 18 2013

Thank you for contacting us. The laboratory that developed and validated the kit uses linear regression but there are some who argue that 4 parameter logisitic nonlinear regression is a better way to curve-fit ELISA data. Either formula is applicab...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"